UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

alpha 4 Integrin is expressed during peripheral nerve regeneration and enhances neurite outgrowth

Vogelezang, MG; Liu, ZQ; Relvas, JB; Raivich, G; Scherer, SS; ffrench-Constant, C; (2001) alpha 4 Integrin is expressed during peripheral nerve regeneration and enhances neurite outgrowth. J NEUROSCI , 21 (17) 6732 - 6744.

Full text not available from this repository.

Abstract

We have shown previously that repair in the peripheral nervous system is associated with a reversion to an embryonic pattern of alternative splicing of the extracellular matrix molecule fibronectin. One of the consequent changes is a relative increase in the number of fibronectins expressing the binding site for alpha4 integrins. Here we show that alpha4 integrins are expressed on dorsal root ganglion neuron cell bodies and growth cones in the sciatic nerve during regeneration and that the interaction of alpha4 integrin with alternatively spliced isoforms of recombinant fibronectins containing the alpha4 binding site enhances neurite outgrowth in dorsal root ganglion neurons. The pheochromocytoma (PC12) neuronal cell line, which normally extends neurites poorly on fibronectin, does so efficiently when alpha4 is expressed in the cells. Experiments using chimeric integrins expressed in PC12 cells show that the alpha4 cytoplasmic domain is necessary and sufficient for this enhanced neurite outgrowth. In both dorsal root ganglion neurons and PC12 cells the alpha4 cytoplasmic domain is tightly linked to the intracellular adapter protein paxillin. These experiments suggest an important role for alpha4 integrin and paxillin in peripheral nerve regeneration and show how alternative splicing of fibronectin may provide a mechanism to enhance repair after injury.

Type: Article
Title: alpha 4 Integrin is expressed during peripheral nerve regeneration and enhances neurite outgrowth
Keywords: integrin, peripheral nerve regeneration, fibronectin, alternative splicing, paxillin, alpha 4, PC12 cell, dorsal root ganglia, chimera, LDV, FACIAL MOTOR NUCLEUS, CELL-LINE PC12, TYROSINE PHOSPHORYLATION, MESSENGER-RNA, FIBRONECTIN ISOFORM, ADHESION SITES, GROWTH-FACTORS, PAXILLIN, MOLECULES, LAMININ
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > Inst for Women's Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > Inst for Women's Health > Maternal and Fetal Medicine
URI: http://discovery.ucl.ac.uk/id/eprint/27612
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item