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Effect of gastrin and anti-gastrin antibodies on proliferation of hepatocyte cell lines.

Caplin, M; Khan, K; Grimes, S; Michaeli, D; Savage, K; Pounder, R; Dhillon, A; (2001) Effect of gastrin and anti-gastrin antibodies on proliferation of hepatocyte cell lines. Dig Dis Sci , 46 (7) pp. 1356-1366.

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Abstract

Gastrin (G-17) and its precursor glycine-extended gastrin (G-17-gly) have been shown to be trophic to some gastrointestinal tumors. This in vitro study assessed the effect of G-17, G-17-gly, anti-gastrin antibodies (anti-G-17), and the CCK-B receptor antagonist PD135,158 on three hepatoma cell lines (PLC/PRF/5, HepG2 and MCA-RH7777) and an embryonic liver cell line (WRL68). The pancreatic adenocarcinoma cell line AR42J was used as a positive control. G-17 and G-17-gly caused significant proliferation of AR42J and WRL68 cell lines. G-17-gly but not G-17 induced significant proliferation of the PLC/PRF/5 cell line. Anti-G-17 and PD135,158 significantly inhibited unstimulated AR42J and WRL68 cell lines. Anti-G-17 also inhibited the proliferative effects of G-17 and G-17-gly on AR42J, WRL68, and PLC/PRF/5 cell lines, whereas PD135,158 inhibited the proliferative effect of G-17 only. G-17 and G-17-gly as well as anti-G-17 and PD135,158 had no effect on HepG2 and MCA-RH77777 cell lines. It is concluded that G-17-stimulated proliferation is mediated via the CCK-B receptor and G-17-gly via a separate, as yet uncharacterized, receptor. There may therefore be a role for gastrin in embryonic hepatocellular proliferation and perhaps also in the proliferation of some hepatocellular tumors.

Type: Article
Title: Effect of gastrin and anti-gastrin antibodies on proliferation of hepatocyte cell lines.
Location: United States
Keywords: Animals, Antibodies, Cell Division, Cell Line, Gastrins, Hepatocytes, Indoles, Liver Neoplasms, Experimental, Meglumine, Receptor, Cholecystokinin B, Receptors, Cholecystokinin
URI: http://discovery.ucl.ac.uk/id/eprint/26163
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