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Effect of gastrin and anti-gastrin antibodies on proliferation of hepatocyte cell lines

Caplin, M; Khan, K; Grimes, S; Michaeli, D; Savage, K; Pounder, R; Dhillon, A; (2001) Effect of gastrin and anti-gastrin antibodies on proliferation of hepatocyte cell lines. Digestive Diseases and Sciences , 46 (7) 1356 - 1366.

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Abstract

Gastrin (G-17) and its precursor glycine-extended gastrin (G-17-gly) have been shown to be trophic to some gastrointestinal tumors. This in vitro study assessed the effect of G-17, G-17-gly, anti-gastrin antibodies (anti-G-17), and the CCK-B receptor antagonist PD135,158 on three hepatoma cell lines (PLC/PRF/5, HepG2 and MCA-RH7777) and an embryonic liver cell line (WRL68). The pancreatic adenocarcinoma cell line AR42J was used as a positive control. G-17 and G-17-gly caused significant proliferation of AR42J and WRL68 cell lines. G-17-gly but not G-17 induced significant proliferation of the PLC/PRF/5 cell line. Anti-G-17 and PD135,158 significantly inhibited unstimulated AR42J and WRL68 cell lines. Anti-G-17 also inhibited the proliferative effects of G-17 and G-17-gly on AR42J, WRL68, and PLC/PRF/5 cell lines, whereas PD135,158 inhibited the proliferative effect of G-17 only. G-17 and G- 17-gly as well as anti-G-17 and PD135,158 had no effect on HepG2 and MCA-RH77777 cell lines. It is concluded that G-17-stimulated proliferation is mediated via the CCK-B receptor and G-17-gly via a separate, as yet uncharacterized, receptor. There may therefore be a role for gastrin in embryonic hepatocellular proliferation and perhaps also in the proliferation of some hepatocellular tumors

Type:Article
Title:Effect of gastrin and anti-gastrin antibodies on proliferation of hepatocyte cell lines
Additional information:UI - 21370761 LA - eng RN - 0 (Antibodies) RN - 0 (CCK-B receptor) RN - 0 (Gastrins) RN - 0 (Indoles) RN - 0 (Receptors, Cholecystokinin) RN - 0 (glycine-extended gastrin 17) RN - 130325-35-8 (PD 135158) RN - 60748-06-3 (gastrin 17) RN - 6284-40-8 (Meglumine) PT - Journal Article DA - 20010731 IS - 0163-2116 SB - AIM SB - IM CY - United States
Keywords:Adenocarcinoma, antagonist, antibodies, Antibody, As, AUTOCRINE GROWTH-STIMULATION, Carcinoma, CCK-B receptor, cell, Cell Line, Cell lines, CELL-LINE, CELL-LINES, Cholecystokinin, COLON-CANCER CELLS, control, effects, embryonic liver, English, GASTRIN, gastrin antibodies, Gastrointestinal, GENE-EXPRESSION, hepatocyte, Hepatoma Cell, in vitro, In Vitro Study, In-vitro, INHIBIT, LINE, LINES, liver, Liver Cell, May, NEW-YORK, positive, PROGASTRIN, proliferation, Receptor, Receptor antagonist, RECEPTOR ANTAGONISTS, Role, Spring, ST, transgenic mice, Tumor, TUMOR-CELLS, TUMORS, USA, VITRO, analogs & derivatives, Animal, antagonists & inhibitors, Cell Division, cytology, drug effects, Gastrins, HEPATOCYTES, IM, immunology, Indoles, LA, Liver Neoplasms, Experimental, Meglumine, pathology, pharmacology, receptors, Receptors, Cholecystokinin, STATE, STATES, united, United States, UNITED-STATES
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Medicine (Division of)

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