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A rapid real-time quantitative polymerase chain reaction for hepatitis B virus

Brechtbuehl, K; Whalley, SA; Dusheiko, GM; Saunders, NA; (2001) A rapid real-time quantitative polymerase chain reaction for hepatitis B virus. Journal of Virological Methods , 93 (1-2) 105 - 113.

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Abstract

Quantification of hepatitis B virus (HBV) DNA in serum is important for monitoring treatment. A rapid and cost effective alternative to the methods available currently was developed based on a real-time quantitative polymerase chain reaction (PCR) done in the LightCycler apparatus. Primers and a probe for sequences of the surface gene of HBV were designed and quantification achieved by reference to standards containing known concentrations of the target sequence. A single copy of the HBV genome could be detected if present in the reaction mixture. The quantitative range of the assay was from 4 x 10(2) to 1.3 x 10(10) surface gene copies/ml serum. Nested PCR was required for quantification in the lower part of this range (<10(5) copies). The real-time PCR and Amplicor Monitor (Roche) tests performed comparably at virus concentrations below 10(6) copies/ml. The commercial test underestimated higher concentrations of virus

Type:Article
Title:A rapid real-time quantitative polymerase chain reaction for hepatitis B virus
Additional information:UI - 21211437 LA - eng RN - 0 (DNA Primers) RN - 0 (DNA, Viral) PT - Journal Article DA - 20010420 IS - 0166-0934 SB - IM CY - Netherlands
Keywords:Amsterdam, ASSAY, Blood, CHAIN, CHAIN-REACTION, Concentration, copy, cost, DEVELOPED, DNA, English, GENE, Genome, HBV, HBV DNA, Hepatitis, hepatitis B, Hepatitis B Virus, Lamivudine, Methods, MONITOR, monitoring, Netherlands, PCR, polymerase, Polymerase Chain Reaction, POLYMERASE-CHAIN-REACTION, PRIMERS, PROBE, QUANTIFICATION, quantitative, RANGE, real-time, rights, Roche, Science, SEQUENCE, SEQUENCES, SERA, serum, SINGLE, Standard, standards, SURFACE, target, TESTS, treatment, TRIAL, VIRUS, analysis, Computer Systems, DNA Primers, Dna, Viral, genetics, health, IM, isolation & purification, LA, Laboratories, public health, Reproducibility of Results, viral, Viral Load
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Medicine (Division of)

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