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IGF-1 regulates cardiac fibroblast apoptosis induced by osmotic stress.

Mockridge, JW; Benton, EC; Andreeva, LV; Latchman, DS; Marber, MS; Heads, RJ; (2000) IGF-1 regulates cardiac fibroblast apoptosis induced by osmotic stress. Biochem Biophys Res Commun , 273 (1) pp. 322-327. 10.1006/bbrc.2000.2934.

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Abstract

In this study we have determined the ability of IGF-1 to protect cardiac fibroblasts against osmotic-induced apoptosis and investigated the potential mechanism(s) underlying this protection. Treatment with IGF-1 (1-100 ng/ml) promoted a dose dependent increase in cell survival against osmotic cell death. Both Akt and ERK1/2 were rapidly phosphorylated by IGF-1 and blocked by wortmannin and PD98059, inhibitors of their upstream activators respectively. However, IGF-1-induced protection was mediated via a wortmannin-dependent but PD98059-independent pathway as determined by cell survival assay suggesting a role of PI3-K/Akt. Furthermore, IGF-1 appeared to reduce the activation of a number of early components in the apoptotic pathway in a wortmannin dependent manner including the osmotic stress-induced perturbation in mitochondrial membrane potential, cleavage and activation of caspase-3 and DNA fragmentation. Thus, the results suggest that IGF-1 regulates osmotic stress-induced apoptosis via the activation of the PI3-K/Akt pathway at a point upstream of the mitochondria and caspase-3.

Type: Article
Title: IGF-1 regulates cardiac fibroblast apoptosis induced by osmotic stress.
Location: United States
DOI: 10.1006/bbrc.2000.2934
Keywords: Animals, Animals, Newborn, Apoptosis, Caspase 3, Caspases, Cell Survival, Cells, Cultured, DNA Fragmentation, Dose-Response Relationship, Drug, Enzyme Activation, Fibroblasts, Insulin-Like Growth Factor I, Isoenzymes, Membrane Potentials, Mitochondria, Mitogen-Activated Protein Kinases, Myocardium, Osmolar Concentration, Peptide Fragments, Phosphatidylinositol 3-Kinases, Phosphorylation, Protein Kinase C, Protein Kinase C-delta, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-akt, Rats, Signal Transduction
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health > ICH - Directors Office
URI: http://discovery.ucl.ac.uk/id/eprint/25144
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