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Intracellular localization of dimethylarginine dimethylaminohydrolase overexpressed in an endothelial cell line

Birdsey, GM; Leiper, JM; Vallance, P; (2000) Intracellular localization of dimethylarginine dimethylaminohydrolase overexpressed in an endothelial cell line. Acta Physiologica Scandinavica , 168 (1) 73 - 79.

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Abstract

Methylarginines are endogenous inhibitors of nitric oxide synthase (NOS) and have been implicated in the regulation of the nitric oxide pathway in health and disease. Cellular concentrations of free methylarginines are determined in part by the activity of dimethylarginine dimethylaminohydrolase (DDAH). There are two isoforms of DDAH which have distinct tissue distributions with some relationship to NOS isoforms. We have determined the intracellular localization of both DDAH isoforms by overexpression of epitope-tagged DDAH in an immortalized endothelial cell line. Immunofluorescence confocal microscopy and immunoblotting indicate that both isoforms are predominantly cytosolic with no specific association with organelles or the plasma membrane. These data suggest that the key role for DDAH may be to ensure that under normal conditions the levels of methylarginines are kept low throughout the whole cell

Type:Article
Title:Intracellular localization of dimethylarginine dimethylaminohydrolase overexpressed in an endothelial cell line
Additional information:UI - 20155896 LA - eng RN - 0 (Epitopes) RN - 0 (Isoenzymes) RN - EC 3. (Hydrolases) RN - EC 3.5.3.18 (dimethylargininase) PT - Journal Article DA - 20000327 IS - 0001-6772 SB - IM CY - ENGLAND JC - 1U4
Keywords:analysis, Association, cell, Cell Line, Cell Line, Transformed, CELL-LINE, Cellular, clinical, confocal microscopy, cytology, Cytosol, disease, distribution, endogenous, Endothelial, endothelial cell, Endothelium, Vascular, enzymology, Epitopes, Fluorescent Antibody Technique, genetics, health, Hydrolases, Immunoblotting, immunofluorescence, INHIBITOR, INHIBITORS, Intracellular Membranes, Isoenzymes, ISOFORMS, LEVEL, localization, May, membrane, metabolism, Microscopy, Microscopy, Confocal, Molecular Sequence Data, nitric oxide, nitric oxide synthase, NITRIC-OXIDE, NOS, OXIDE, pharmacology, plasma, Plasma membrane, regulation, Sequence Tagged Sites, Support, Non-U.S.Gov't, SYNTHASE, therapeutic, Tissue, Tissue Distribution, UK
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Medicine (Division of)

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