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The major phosphorylation site of the NADPH oxidase component p67(phox) is Thr<sup>233</sup>

Forbes, LV; Truong, O; Wientjes, FB; Moss, SJ; Segal, AW; (1999) The major phosphorylation site of the NADPH oxidase component p67(phox) is Thr<sup>233</sup>. Biochemical Journal , 338 (1) pp. 99-105. 10.1042/0264-6021:3380099.

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Abstract

Phosphorylation of p67(phox) was shown to increase two- to three-fold upon stimulation by PMA, N-formylmethionyl-leucyl-phenylalanine or serum-opsonized zymosan. Phosphopeptide mapping showed one major tryptic peptide for p67(phox) immunoprecipitated from resting or stimulated cells. In vitro phosphorylation of p67(phox) by isolated cytosol or mitogenactivated protein kinase also generated the same phosphopeptide. Results of cyanogen bromide digestion and HPLC-MS suggested that Thr233 was the phosphorylated residue. Mutagenesis of Thr233 to alanine resulted in loss of phosphorylation in vitro. In the present work, Thr233 has been identified as the major phosphorylation site of p67(phox), which is situated in a proline-rich domain.

Type: Article
Title: The major phosphorylation site of the NADPH oxidase component p67(phox) is Thr<sup>233</sup>
DOI: 10.1042/0264-6021:3380099
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Internal Medicine
URI: http://discovery.ucl.ac.uk/id/eprint/22393
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