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Presence of constitutive endothelial nitric oxide synthase immunoreactivity in urothelial cells of hamster proximal urethra.

Pinna, C; Eberini, I; Puglisi, L; Burnstock, G; (1999) Presence of constitutive endothelial nitric oxide synthase immunoreactivity in urothelial cells of hamster proximal urethra. Eur J Pharmacol , 367 (1) pp. 85-89.

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Abstract

Electrical field stimulation caused frequency-dependent relaxations in precontracted strips of hamster proximal urethra, which were attenuated by L-N(G)-nitroarginine methyl ester (10(-4) M) and completely blocked by tetrodotoxin (10(-6) M). Strips of hamster urethra devoid of urothelium showed reduced relaxant responses to electrical field stimulation which were abolished by L-N(G)-nitroarginine methyl ester (10(-4) M). Western blot analysis showed the presence of a constitutive endothelial nitric oxide synthase in the urothelial layer, suggesting that urothelium may release nitric oxide in response to electrical field stimulation and that this release is blocked by tetrodotoxin. It is suggested that the urothelium may contribute to relaxations of the smooth muscle of hamster urethra produced by nerve stimulation.

Type: Article
Title: Presence of constitutive endothelial nitric oxide synthase immunoreactivity in urothelial cells of hamster proximal urethra.
Location: Netherlands
Keywords: Animals, Arginine, Blotting, Western, Cricetinae, Electric Stimulation, Enzyme Inhibitors, In Vitro Techniques, Male, Mesocricetus, Muscle Contraction, Muscle Relaxation, Muscle, Smooth, NG-Nitroarginine Methyl Ester, Nitric Oxide Synthase, Nitric Oxide Synthase Type III, Tetrodotoxin, Urethra, Urothelium
URI: http://discovery.ucl.ac.uk/id/eprint/22273
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