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P2X receptors in the rat duodenal villus.

Gröschel-Stewart, U; Bardini, M; Robson, T; Burnstock, G; (1999) P2X receptors in the rat duodenal villus. Cell Tissue Res , 297 (1) pp. 111-117.

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Abstract

Immunohistochemical techniques were performed on freshly frozen sections of the duodenum of the rat using specific polyclonal antibodies to unique peptide sequences of P2X1-7 receptors. Of the antibodies to the seven known P2X receptor subtypes that mediate extracellular signalling by nucleotides, three reacted with discrete structures in the duodenal villus of the rat. Anti-P2X1 reacted with the capillary plexus in the intestinal villus, which did not extend to the crypt region, suggesting that nucleotides may be involved in the uptake and transport of metabolites. Anti-P2X5 immunostained the membranes of the narrow "stem" of villus goblet cells, where the nucleus and cell organelles reside, possibly influencing synthesis and release of mucins. P2X7 receptor immunoreactivity was only seen in the membranes of enterocytes and goblet cells at the tip of the villus, where cells are exfoliated into the lumen, consistent with earlier findings that P2X7 is involved in apoptotic events. Thus, in complex structures such as the intestinal villus, purinoceptors appear to participate in several and diverse signalling functions.

Type: Article
Title: P2X receptors in the rat duodenal villus.
Location: Germany
Keywords: Amino Acid Sequence, Animals, Antibody Specificity, Duodenum, Epitopes, Female, Immunoglobulin G, Intestinal Mucosa, Microvilli, Molecular Sequence Data, Muscle, Smooth, Muscle, Smooth, Vascular, Rats, Rats, Sprague-Dawley, Receptors, Purinergic P2
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
URI: http://discovery.ucl.ac.uk/id/eprint/22242
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