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Budding of filamentous and non-filamentous influenza A virus occurs via a VPS4 and VPS28-independent pathway

Bruce, EA; Medcalf, L; Crump, CM; Noton, SL; Stuart, AD; Wise, HM; Elton, D; ... Digard, P; + view all (2009) Budding of filamentous and non-filamentous influenza A virus occurs via a VPS4 and VPS28-independent pathway. VIROLOGY , 390 (2) 268 - 278. 10.1016/j.virol.2009.05.016.

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Abstract

The mechanism of membrane scission during influenza A virus budding has been the subject of controversy. We confirm that influenza M1 binds VPS28, a subunit of the ESCRT-1 complex. However, confocal microscopy of infected cells showed no marked colocalisation between M1 and VPS28 or VPS4 ESCRT proteins, or relocalisation of the cellular proteins. Trafficking of HA and M1 appeared normal when endosomal sorting was impaired by expression of inactive VPS4. Overexpression of either isoform of VPS28 or wildtype or dominant negative VPS4 proteins did not alter production of filamentous virions. SiRNA depletion of endogenous VPS28 had no significant effect on influenza virus replication. Furthermore, cells expressing wildtype or dominant-negative VPS4 replicated filamentous and non-filamentous strains of influenza to similar titres, indicating that influenza release is VPS4-independent. Overall, we see no role for the ESCRT pathway in influenza virus budding and the significance of the M1-VPS28 interaction remains to be determined. (C) 2009 Elsevier Inc. All rights reserved.

Type: Article
Title: Budding of filamentous and non-filamentous influenza A virus occurs via a VPS4 and VPS28-independent pathway
DOI: 10.1016/j.virol.2009.05.016
Keywords: Assembly, ESCRT, VPS28-1, VPS28-2, Multivesicular body, PROTEIN SORTING PATHWAY, RETRACTED ARTICLE. SEE, MATRIX PROTEIN, ESCRT-I, ACTIN CYTOSKELETON, VIRAL MORPHOLOGY, NUCLEAR EXPORT, M1, DOMAINS, NUCLEOPROTEIN
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Security and Crime Science
URI: http://discovery.ucl.ac.uk/id/eprint/191756
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