Rachel, RA;
Dolen, G;
Hayes, NL;
Lu, A;
Erskine, L;
Nowakowski, RS;
Mason, CA;
(2002)
Spatiotemporal features of early neuronogenesis differ in wild-type and albino mouse retina.
Journal of Neuroscience
, 22
(11)
4249 - 4263.
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Abstract
In albino mammals, lack of pigment in the retinal pigment epithelium is associated with retinal defects, including poor visual acuity from a photoreceptor deficit in the central retina and poor depth perception from a decrease in ipsilaterally projecting retinal fibers. Possible contributors to these abnormalities are reported delays in neuronogenesis (Ilia and Jeffery, 1996) and retinal maturation (Webster and Rowe, 1991). To further determine possible perturbations in neuronogenesis and/or differentiation, we used cell-specific markers and refined birth dating methods to examine these events during retinal ganglion cell (RGC) genesis in albino and pigmented mice from embryonic day 11 (E11) to E18. Our data indicate that relative to pigmented mice, more ganglion cells are born in the early stages of neuronogenesis in the albino retina, although the initiation of RGC genesis in the albino is unchanged. The cellular organization of the albino retina is perturbed as early as E12. In addition, cell cycle kinetics and output along the nasotemporal axis differ in retinas of albino and pigmented mice, both absolutely, with the temporal aspect of the retina expanded in albino, and relative to the position of the optic nerve head. Finally, blocking melanin synthesis in pigmented eyecups in culture leads to an increase in RGC differentiation, consistent with a role for melanin formation in regulating RGC neuronogenesis. These results point to spatiotemporal defects in neuronal production in the albino retina, which could perturb expression of genes that specify cell fate, number, and/or projection phenotype
| Type: | Article |
|---|---|
| Title: | Spatiotemporal features of early neuronogenesis differ in wild-type and albino mouse retina |
| Open access status: | An open access version is available from UCL Discovery |
| Language: | English |
| Additional information: | UI - 22035660 DA - 20020531 IS - 1529-2401 LA - eng PT - Journal Article RN - 0 (Eye Proteins) RN - 0 (Homeodomain Proteins) RN - 0 (Melanins) RN - 0 (biotinylated dextran amine) RN - 0 (insulin gene enhancer binding protein Isl-1) RN - 58-85-5 (Biotin) RN - 59-14-3 (Bromodeoxyuridine) RN - 9004-54-0 (Dextrans) RN - EC 1.14.18.1 (Monophenol Monooxygenase) SB - IM SB - S This work is licensed under the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. The license allows you to copy, distribute, and transmit the work, as well as adapting it. However, you must attribute the work to the author (but not in any way that suggests that they endorse you or your use of the work), and cannot use the work for commercial purposes without prior permission of the author. If you alter or build upon this work, you can distribute the resulting work only under the same or similar license to this one. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ or send a letter to Creative Commons, 444 Castro Street, Suite 900, Mountain View, California, 94041, USA. |
| Keywords: | 1996, abnormalities, ABNORMALITY, Albinism, analogs & derivatives, Animal, antagonists & inhibitors, As, Axis, BINDING, BINDING PROTEIN, BINDING-PROTEIN, biosynthesis, Biotin, BIRTH, BORN, Bromodeoxyuridine, cell, Cell Count, Cell Cycle, Cell Differentiation, Cell Division, CELL-CYCLE, CELLS, Cellular, culture, cycle, cytology, dating, DEFECT, DEFECTS, deficiency, delay, Depth, Dextrans, differentiation, EC, embryology, EPITHELIA, Epithelium, EVENTS, expression, Eye, Eye Proteins, FATE, FEATURES, FIBERS, Flow Cytometry, formation, ganglion, GENE, Genes, genesis, Head, Homeodomain Proteins, Ilium, IM, Immunohistochemistry, in vitro, INCREASE, INDICATE, INITIATION, Insulin, kinetic, Kinetics, LA, mammal, Mammals, MARKER, Markers, maturation, Melanins, Methods, mice, Mice, Inbred C57BL, Mutant Strains, Monooxygenase, Monophenol Monooxygenase, mouse, nerve, neurons, NUMBER, Optic Nerve, ORGANIZATION, OUTPUT, pathology, perception, Phenotype, physiology, PIGMENT EPITHELIUM, poor, POSITION, production, PROTEIN, Proteins, Regulating, Relative, Result, retina, retinal ganglion cells, S, S Phase, Species Specificity, Support, Non-U.S.Gov't, U.S.Gov't, Non-P.H.S., P.H.S., synthesis, visual, visual acuity, WILD-TYPE |
| URI: | https://discovery.ucl.ac.uk/id/eprint/191239 |
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