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Spectrophotometry for cerebrospinal fluid pigment analysis

Petzold, A.; Sharpe, L.T.; Keir, G.; (2006) Spectrophotometry for cerebrospinal fluid pigment analysis. Neurocritical care , 4 (2) pp. 153-62. 10.1385/NCC:4:2:153. Green open access


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The use of spectrophotometry for the analysis of the cerebrospinal fluid (CSF) is reviewed. The clinically relevant CSF pigments--oxyhemoglobin and bilirubin--are introduced and discussed with regard to clinical differential diagnosis and potentially confounding variables (the four T's: traumatic tap, timing, total protein, and total bilirubin). The practical laboratory aspects of spectrophotometry and automated techniques are presented in the context of analytical and clinical specificity and sensitivity. The perceptual limitations of human color vision are highlighted and the use of visual assessment of the CSF is discouraged in light of recent evidence from a national audit in the United Kingdom. Finally, future perspectives including the need for longitudinal CSF profiling and routine spectrophotometric calibration are outlined.

Type: Article
Title: Spectrophotometry for cerebrospinal fluid pigment analysis
Open access status: An open access version is available from UCL Discovery
DOI: 10.1385/NCC:4:2:153
Publisher version: http://dx.doi.org/10.1385/NCC:4:2:153
Language: English
Additional information: The original publication is available at www.springerlink.com
Keywords: Spectrophotometry, xanthochromia, cerebrospinal fluid, subarachnoid hemorrhage
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Neuroinflammation
URI: http://discovery.ucl.ac.uk/id/eprint/18934
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