Monot, M and Honore, N and Garnier, T and Zidane, N and Sherafi, D and Paniz-Mondolfi, A and Matsuoka, M and Taylor, GM and Donoghue, HD and Bouwman, A and Mays, S and Watson, C and Lockwood, D and Khamispour, A and Dowlati, Y and Shen, JP and Rea, TH and Vera-Cabrera, L and Stefani, MM and Banu, S and Macdonald, M and Sapkota, BR and Spencer, JS and Thomas, J and Harshman, K and Singh, P and Busso, P and Gattiker, A and Rougemont, J and Brennan, PJ and Cole, ST (2009) Comparative genomic and phylogeographic analysis of Mycobacterium leprae. NAT GENET , 41 (12) 1282 - U39. 10.1038/ng.477.
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Abstract
Reductive evolution and massive pseudogene formation have shaped the 3.31-Mb genome of Mycobacterium leprae, an unculturable obligate pathogen that causes leprosy in humans. The complete genome sequence of M. leprae strain Br4923 from Brazil was obtained by conventional methods (6 x coverage), and Illumina resequencing technology was used to obtain the sequences of strains Thai53 (38 x coverage) and NHDP63 (46 x coverage) from Thailand and the United States, respectively. Whole-genome comparisons with the previously sequenced TN strain from India revealed that the four strains share 99.995% sequence identity and differ only in 215 polymorphic sites, mainly SNPs, and by 5 pseudogenes. Sixteen interrelated SNP subtypes were defined by genotyping both extant and extinct strains of M. leprae from around the world. The 16 SNP subtypes showed a strong geographical association that reflects the migration patterns of early humans and trade routes, with the Silk Road linking Europe to China having contributed to the spread of leprosy.
| Type: | Article |
|---|---|
| Title: | Comparative genomic and phylogeographic analysis of Mycobacterium leprae |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/ng.477 |
| Keywords: | NUMBER TANDEM REPEATS, HELICOBACTER-PYLORI, LEPROSY PATIENTS, YERSINIA-PESTIS, CAUSATIVE AGENT, BURULI ULCER, TUBERCULOSIS, SEQUENCE, EVOLUTION, POPULATIONS |
| UCL classification: | UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Infection and Immunity (Division of) > Research Department of Infection |
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