Springett, R; Newman, J; Cope, M; Delpy, DT; (2000) Oxygen dependency and precision of cytochrome oxidase signal from full spectral NIRS of the piglet brain. Am J Physiol Heart Circ Physiol , 279 (5) H2202 - H2209.
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Oxidation changes of the copper A (Cu(A)) center of cytochrome oxidase in the brain were measured during brief anoxic swings at both normocapnia and hypercapnia (arterial PCO(2) approximately 55 mmHg). Hypercapnia increased total hemoglobin from 37.5 +/- 9.1 to 50.8 +/- 12.9 micromol/l (means +/- SD; n = 7), increased mean cerebral saturation (Smc(O(2))) from 65 +/- 4 to 77 +/- 3%, and oxidized Cu(A) by 0.43 +/- 0.23 micromol/l. During the onset of anoxia, there were no significant changes in the Cu(A) oxidation state until Smc(O(2)) had fallen to 43 +/- 5 and 21 +/- 6% at normocapnia and hypercapnia, respectively, and the maximum reduction during anoxia was not significantly different at hypercapnia (1.49 +/- 0.40 micromol/l) compared with normocapnia (1.53 +/- 0.44 micromol/l). Residuals of the least squares fitting algorithm used to convert near-infrared spectra to concentrations are presented and shown to be small compared with the component of attenuation attributed to the Cu(A) signal. From these observations, we conclude that there is minimal interference between the hemoglobin and Cu(A) signals in this model, the Cu(A) oxidation state is independent of cerebral oxygenation at normoxia, and the oxidation after hypercapnia is not the result of increased cerebral oxygenation.
|Title:||Oxygen dependency and precision of cytochrome oxidase signal from full spectral NIRS of the piglet brain.|
|Keywords:||Animals, Blood Pressure, Brain, Copper, Electron Transport Complex IV, Heart Rate, Hemoglobins, Hypercapnia, Hypoxia, Brain, Male, Oxidation-Reduction, Oxygen, Oxyhemoglobins, Regression Analysis, Reproducibility of Results, Spectroscopy, Near-Infrared, Swine|
|UCL classification:||UCL > School of BEAMS > Faculty of Engineering Science > Medical Physics and Bioengineering|
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