UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

The role of chloride ions in the regulation of steroidogenesis in rat Leydig cells and adrenal cells

Cooke, BA; Ashford, L; Abayasekara, DR; Choi, M; (1999) The role of chloride ions in the regulation of steroidogenesis in rat Leydig cells and adrenal cells. The Journal of Steroid Biochemistry and Molecular Biology , 69 (1) pp. 359-365.

Full text not available from this repository.

Abstract

The role of chloride ions in the regulation of steroidogenesis in rat Leydig cells and adrenal cells has been investigated. It was found that the chloride channel blocker 4,4'-diisothiocyanatostilbene-2,2'- disulphonic acid (DIDS) inhibited LH but not dibutyryl cAMP (dbcAMP)- stimulated steroidogenesis in the Leydig cells. This was found to be via an inhibition of cAMP production, because both LH- and forskolin- stimulated cAMP productions were inhibited by DIDS. The exclusion of chloride ions enhanced steroidogenesis during incubation of Leydig cells and adrenal cells with dbcAMP. The adrenal cells were found to be more sensitive to dbcAMP than Leydig cells and the enhancing effects of chloride removal were higher. In the presence of chloride ions, near maximum steroidogenesis was achieved with approximately 60 microM and 1 mM dbcAMP in the adrenal and Leydig cells, respectively. In the absence of chloride ions the concentrations required decreased approximately 50- fold and 10-fold, respectively. It is concluded that although LH may regulate DIDS sensitive chloride channels, the enhanced stimulation of cAMP-mediated steroidogenesis by chloride exclusion is not mediated via these channels. We propose a model based on the present and previous studies [1] with Leydig tumour (MA10) cells i.e. that intracellular chloride ion depletion enhances the action of cAMP on protein synthesis which results in increased synthesis of the Steroidogenic Acute Regulator (StAR) protein and consequently increased steroidogenesis

Type: Article
Title: The role of chloride ions in the regulation of steroidogenesis in rat Leydig cells and adrenal cells
Additional information: UI - 99345381 LA - Eng RN - 0 (Calcium Channel Blockers) RN - 0 (Chlorides) RN - 53005-05-3 (DIDS) RN - 57-85-2 (Testosterone) RN - 60-92-4 (Cyclic AMP) PT - JOURNAL ARTICLE DA - 19990802 IS -0960-0760 SB - M SB - X CY - ENGLAND JC - AX4 AA - Author EM -199910
Keywords: regulation, rat, CELLS, Calcium, AMP, Adrenal Glands, Animal, Biochemistry, biosynthesis, Calcium Channel Blockers, cell, Chlorides, Cyclic AMP, cytology, DIDS, drug effects, Forskolin, Leydig Cells, Male, metabolism, Molecular Biology, pharmacology, physiology, rats, Testosterone, Acids, ANS, Proteins, synthesis
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/186616
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item