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Oct-1 POU and octamer DNA co-operate to recognise the Bob-1 transcription co-activator via induced folding

Chang, JF; Phillips, K; Lundback, T; Gstaiger, M; Ladbury, JE; Luisi, B; (1999) Oct-1 POU and octamer DNA co-operate to recognise the Bob-1 transcription co-activator via induced folding. Journal of Molecular Biology , 288 (5) pp. 941-952.

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Abstract

The expression of immunoglobulin genes is controlled in part by the DNA- binding protein Oct-1 and the B cell-specific transcription co- activator, Bob1 (also known as OCA-B or OBF-1) that together form a complex on the Igkappa promoter. We have characterised the assembly of the ternary complex using biophysical methods. Bob1 binds specifically as a monomer to the complex of the Oct-1 DNA-binding domain (Oct-1 POU) and the Igkappa promoter, but binds weakly to either Oct-1 POU or the Igkappa promoter alone, indicating that both are required to make an avid complex. Ternary complex formation requires a defined DNA sequence, as the stability of the complex can be strongly affected by a single base-pair change or by removing 5-methyl groups from selected thymine bases.In isolation, Bob1 appears to have little secondary structure, but may become partially structured upon recruitment into the ternary complex as demonstrated by circular dichroism spectra and calorimetry. These and other findings suggest that ternary complex formation requires a defined geometry of the POU/DNA complex, and that the co-activator makes stereo-specific contacts to both the POU protein and the major groove of the DNA that induces its fold. Copyright 1998 Academic Press

Type: Article
Title: Oct-1 POU and octamer DNA co-operate to recognise the Bob-1 transcription co-activator via induced folding
Additional information: UI - 99264415 LA - Eng RN - 0 (octamer transcription factor OTF-1) RN - 0 (Bob1 protein) RN - 0 (DNA-Binding Proteins) RN - 0 (Recombinant Fusion Proteins) RN - 0 (Trans-Activators) RN - 0 (Transcription Factors) PT - JOURNAL ARTICLE DA - 19990625 IS -0022-2836 SB - M SB - X CY - ENGLAND JC - J6V AA - Author EM -199909
Keywords: DNA, Proteins, recombinant, Recombinant Fusion Proteins, Base Sequence, Biochemistry, Bob1 protein, Calorimetry, Circular Dichroism, Crystallography, X-Ray, DNA Binding Proteins, DNA-Binding Proteins, GENE, Genes, Kinetics, Methods, Models, Genetic, Models, Molecular, octamer transcription factor OTF 1, physiology, Protein Folding, Protein Structure, Secondary, Support, Non-U.S.Gov't, Thermodynamics, Time Factors, Trans Activators, Trans-Activators, Transcription Factors, expression, ANS, Structure
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/186613
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