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Insulin activates the alpha isoform of class II phosphoinositide 3- kinase

Brown, RA; Domin, J; Arcaro, A; Waterfield, MD; Shepherd, PR; (1999) Insulin activates the alpha isoform of class II phosphoinositide 3- kinase. Journal of Biological Chemistry , 274 (21) pp. 14529-14532. Gold open access

Abstract

The novel class II phosphoinositide (PI) 3-kinases are characterized by the presence of a C-terminal C2 domain, but little is known about their regulation. We find insulin causes a rapid 2-3-fold increase in the activity of PI 3-kinase C2alpha (PI3K-C2alpha) in CHO-IR cells, 3T3-L1 adipocytes, and fully differentiated L5L6 myotubes. No insulin-induced activation of PI3K-C2alpha was observed in cell types known to have low responsiveness to insulin including HEK 293 cells, 3T3-L1 preadipocytes, and undifferentiated L5L6 myoblasts. The mechanism of activation of PI3K-C2alpha by insulin differs from that of class Ia PI 3-kinases in that insulin stimulation did not cause PI3K-C2alpha to associate with IRS-1 or insulin receptor. PI3K-C2alpha existed as a doublet, and insulin stimulation caused a redistribution from the lower molecular weight band to the higher molecular weight band, suggesting phosphorylation-induced bandshift. Consistent with this, in vitro phosphatase treatment reduced the intensity of the upper band back to that seen in unstimulated cells. This suggests that insulin-induced phosphorylation could play a role in regulation of the activity of PI3K- C2alpha. The finding that insulin activates PI3K-C2alpha in cell types known to possess a wide range of responses to insulin suggests that PI3K-C2alpha is a novel component of insulin-stimulated signaling cascades

Type: Article
Title: Insulin activates the alpha isoform of class II phosphoinositide 3- kinase
Open access status: An open access publication
Additional information: UI - 99262593 LA - Eng RN - EC 2.7.1.137 (1-Phosphatidylinositol 3-Kinase) RN - 0 (Protein Isoforms) RN - 11061-68-0 (Insulin) PT -JOURNAL ARTICLE DA - 19990709 IS - 0021-9258 SB - M SB - X CY -UNITED STATES JC - HIV AA - Author EM - 199909
Keywords: Proteins, 1 Phosphatidylinositol 3 Kinase, 1-Phosphatidylinositol 3-Kinase, adipocyte, Adipocytes, Animal, Biochemistry, cell, CELLS, Hiv, In Vitro, Insulin, metabolism, Mice, Molecular Biology, Molecular Weight, phosphoinositide, Phosphorylation, physiology, PI 3-kinase, Protein Isoforms, Support, Non-U.S.Gov't, regulation, Receptor
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/186612
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