Human sperm proteome: immunodominant sperm surface antigens identified with sera from infertile men and women.
Biology of Reproduction
61 - 69.
The objective of this study was to identify those immunodominant sperm antigens recognized by antisperm antibodies (ASA) in the serum samples of infertile men and women. High-resolution two-dimensional gel electrophoresis was employed to separate human sperm proteins using isoelectric focusing or nonequilibrium pH gradient electrophoresis, followed by PAGE. Serum samples from 15 infertile male subjects and 6 infertile female subjects that contained ASA as assayed by the immunobead binding test (IBT) were analyzed by Western blotting followed by enhanced chemiluminescence (ECL). Serum samples from 10 fertile subjects (5 males and 5 females) that were ASA negative by IBT were used as controls. The ECL blots were analyzed by computer scanning to compare the immunoreactivity between serum samples from fertile and infertile subjects and to identify the antigens unique to the sera of the infertile subjects; 98 sperm
|Title:||Human sperm proteome: immunodominant sperm surface antigens identified with sera from infertile men and women|
|Additional information:||UI - 99306885 LA - Eng RN - 0 (Antigens, Surface) RN - 0 (IgG) RN - 0 (IgM) RN - 0 (Immune Sera) RN - 0 (Proteins) PT - JOURNAL ARTICLE ID - D43/TW/HD 00654/TW/FIC ID - U54 29099 DA - 19990903 IS - 0006-3363 SB - M CY - UNITED STATES JC - A3W AA - Author EM -199911|
|Keywords:||sperm, Proteome, Antigens, IgG, Proteins, Id, United States, analysis, Antibodies, antibody, Antigens, Surface, blood, Blotting, Western, Chemiluminescence, Electrophoresis, Polyacrylamide Gel, Female, Hydrogen-Ion Concentration, IgM, Immune Sera, immunology, Infertility, Female, Infertility, Male, Isoelectric Focusing, Male, Sex Characteristics, Spermatozoa, Support, Non-U.S.Gov't, Support, U.S.Gov't, P.H.S., pH, control|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of)|
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