Page, MJ and Amess, B and Townsend, RR and Parekh, R and Herath, A and Brusten, L and Zvelebil, MJ and Stein, RC and Waterfield, MD and Davies, SC and O'Hare, MJ (1999) Proteomic definition of normal human luminal and myoepithelial breast cells purified from reduction mammoplasties. Proceedings of the National Academy of Sciences of the United States of America , 96 (22) 12589 - 12594.
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Normal human luminal and myoepithelial breast cells separately purified from a set of 10 reduction mammoplasties by using a double antibody magnetic affinity cell sorting and Dynabead immunomagnetic technique were used in two-dimensional gel proteome studies. A total of 43,302 proteins were detected across the 20 samples, and a master image for each cell type comprising a total of 1,738 unique proteins was derived. Differential analysis identified 170 proteins that were elevated 2-fold or more between the two breast cell types, and 51 of these were annotated by tandem mass spectrometry. Muscle-specific enzyme isoforms and contractile intermediate filaments including tropomyosin and smooth muscle (SM22) alpha protein were detected in the myoepithelial cells, and a large number of cytokeratin subclasses and isoforms characteristic of luminal cells were detected in this cell type. A further 134 nondifferentially regulated proteins were also annotated from the two breast cell types, making this the most extensive study to date of the protein expression map of the normal human breast and the basis for future studies of purified breast cancer cells
|Title:||Proteomic definition of normal human luminal and myoepithelial breast cells purified from reduction mammoplasties|
|Additional information:||UI - 20006278 LA - Eng RN - 0 (Proteome) PT - JOURNAL ARTICLE DA - 19991210 IS - 0027-8424 SB - M SB - X CY - UNITED STATES JC -PV3 AA - Author EM - 200002|
|Keywords:||Breast, cell, CELLS, Luminal, Mammoplasties, Myoepithelial, reduction, 2 DIMENSIONAL ELECTROPHORESIS, AFFINITIES, AFFINITY, analysis, ANNEXIN II, Antibodies, antibody, Breast, Breast cancer, cancer, DIFFERENTIAL EXPRESSION, Epithelial Cells, expression, extensive, GEL ELECTROPHORESIS, HUMAN BREAST, HUMAN MAMMARY GLAND, image, IMMUNOMAGNETIC SEPARATION, muscle, POLYPEPTIDE DIFFERENCES, PROTEIN, PROTEIN EXPRESSION, Proteins, Proteome, smooth muscle, Technique, TWO DIMENSIONAL ELECTROPHORESIS, United States, Adult, cytology, Electrophoresis, Gel, Two-Dimensional, Female, Mammaplasty, metabolism, Middle Age, Spectrum Analysis, Mass, Enzymes|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of)|
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Surgery and Interventional Science (Division of) > Research Department of General Surgery
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