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Regulation of the myelin gene periaxin provides evidence for Krox-20-independent myelin-related signalling in Schwann cells

Parkinson, DB; Dickinson, S; Bhaskaran, A; Kinsella, MT; Brophy, PJ; Sherman, DL; Sharghi-Namini, S; ... Jessen, KR; + view all (2003) Regulation of the myelin gene periaxin provides evidence for Krox-20-independent myelin-related signalling in Schwann cells. MOL CELL NEUROSCI , 23 (1) 13 - 27. 10.1016/S1044-7431(03)00024-1.

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Abstract

We investigated the role of Krox-20 (Egr2), a transcription factor that regulates myelination, in controlling the myelin-associated protein periaxin. In developing Schwarm cells, periaxin immunoreactivity appeared at least 2 days before Krox-20-immunopositive nuclei. Consistent with this, in Krox-20 null mice periaxin was upregulated on schedule, albeit to a lower level. In culture Krox-20 and periaxin were upregulated by CAMP as expected for myelin genes. Only those cells with the highest periaxin levels also expressed Krox-20, while other periaxin-positive cells remained Krox-20-negative. Furthermore, CAMP elevated periaxin even in Krox-20 null cells. We also found that in culture enforced Krox-20 expression induced expression of periaxin mRNA and protein in the absence of CAMP elevating agents, and that this induction was inhibited by the co-repressor NAB2. These findings reveal a dual mechanism for periaxin regulation and suggest that the role of Krox-20 is to amplify an earlier Krox-20-independent activation of the periaxin gene. Thus the axonal signals responsible for myelination are only partially transduced in Schwarm cells by mechanisms that depend on Krox-20. (C) 2003 Elsevier Science (USA). All rights reserved.

Type: Article
Title: Regulation of the myelin gene periaxin provides evidence for Krox-20-independent myelin-related signalling in Schwann cells
DOI: 10.1016/S1044-7431(03)00024-1
Keywords: N-CAM, MONOCLONAL-ANTIBODIES, DEVELOPING HINDBRAIN, EMBRYONIC NERVES, NAB PROTEINS, A EGR-1, KROX-20, DIFFERENTIATION, EXPRESSION, LOCALIZATION
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: http://discovery.ucl.ac.uk/id/eprint/185756
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