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Spinal microglia and neuropathic pain in young rats

Moss, A; Beggs, S; Vega-Avelaira, D; Costigan, M; Hathway, GJ; Salter, MW; Fitzgerald, M; (2007) Spinal microglia and neuropathic pain in young rats. PAIN , 128 (3) 215 - 224. 10.1016/j.pain.2006.09.018.

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Abstract

Neuropathic pain behaviour is not observed in neonatal rats and tactile allodynia does not develop in the spared nerve injury (SNI) model until rats are 4 weeks of age at the time of surgery. Since activated spinal microglia are known to play a key role in neuropathic pain, we have investigated whether the microglial response to nerve injury in young rats differs from that in adults. Here we show that dorsal horn microglial activation, visualised with IBA-1 immunostaining, is significantly less in postnatal day (P) 10 rat pups than in adults, 7 days after SNI. This was confirmed by qPCR analysis of IBA-1 mRNA and mRNA of other microglial markers, integrin-alpha M, MHC-II DM alpha and MHC-II DM beta. Dorsal horn IBA-1+ve microglia could be activated, however, by intraspinal injections of lipopolysaccharide (LPS) or N-methyl-D-aspartate (NMDA) at P10, although the increase in the levels of mRNA for all microglial markers was less than in the adult rat. In addition, P10 rats developed a small but significant mechanical allodynia in response to intrathecal LPS. Intrathecal injection of cultured ATP-activated microglia, known to cause mechanical allodynia in adult rats, had no behavioural effect at P10 and only began to cause allodynia if injections were performed at P16. The results clearly demonstrate immaturity of the microglial response triggered by nerve injury in the first postnatal weeks which may explain the absence of tactile allodynia following peripheral nerve injury in young rats. (c) 2006 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.

Type: Article
Title: Spinal microglia and neuropathic pain in young rats
DOI: 10.1016/j.pain.2006.09.018
Keywords: microglia, Iba-1, lipopolysaccharide, neuropathic pain, neonatal, dorsal horn, PERIPHERAL-NERVE INJURY, DORSAL-HORN, POSTNATAL-DEVELOPMENT, ADULT-RAT, MECHANICAL ALLODYNIA, CENTRAL TERMINALS, GLIAL ACTIVATION, CORD, MACROPHAGES, RESPONSES
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health > ICH Developmental Neurosciences Prog
URI: http://discovery.ucl.ac.uk/id/eprint/182810
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