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Expression of IL-4 mRNA in peripheral blood mononuclear cells from normal donors in relation to expression of TLR2

Chang, JS; Dheda, K; Huggett, JF; Kim, LU; Zumla, A; Rook, GAW; (2006) Expression of IL-4 mRNA in peripheral blood mononuclear cells from normal donors in relation to expression of TLR2. IMMUNOL LETT , 106 (2) 194 - 197. 10.1016/j.imlet.2006.06.001.

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Abstract

When IL-4 mRNA was distinguished from mRNA encoding its antagonist, the splice variant IL-4 delta 2, it was found to correlate directly with expression of TLR2 in fresh peripheral blood mononuclear cells (PBMCs) from normal donors (p=0.0013). Similarly IL-4 mRNA was high when TLR2 mRNA was abundant compared to levels of mRNA encoding its heterodimerisation partners TLR1 (p = 0.0007) or TLR6 (p = 0.0007). IL-4 delta 2 tended to show the reverse effect; IL-4 delta 2 mRNA was high when TLR2 was low relative to TLR 1 (p = 0.001). When subpopulations of the PBMCs were examined these relationships were found to be restricted to the CD3+ cells. The CD3+ cell population from 5 of 10 donors had detectable TLR2 mRNA. When levels of TLR1, IL-4 and IFN-gamma mRNA were assayed in the TLR2(low) and TLR2(high) CD3+ cells, it was found that IL-4 mRNA was restricted to the TLR2 high T cells (p = 0.007) while TLR I was higher in the TLR2(low) T cells (p = 0.015). IFN-gamma was also somewhat increased in the TLR2(low) (ns). None of these correlations with TLR mRNA expression levels were found in similar samples from tuberculosis patients, or when similar analyses were performed with data for IL-10 mRNA in cells from the same donors. We conclude that in T cell populations from normal donors, expression of IL-4 (but not of its antagonist, IL-4 delta 2, or of IL-10) is associated with high TLR2 and low TLR1. (c) 2006 Published by Elsevier B.V.

Type:Article
Title:Expression of IL-4 mRNA in peripheral blood mononuclear cells from normal donors in relation to expression of TLR2
DOI:10.1016/j.imlet.2006.06.001
Keywords:TLR2, IL-4, IL-4 delta 2, Th1, Th2, IL-10, TOLL-LIKE RECEPTOR-2, REGULATORY T-CELLS, CUTTING EDGE, PULMONARY TUBERCULOSIS, IMMUNE-RESPONSE, IN-VITRO, LIPOPROTEINS, ACTIVATION
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Infection and Immunity (Division of) > Research Department of Infection
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Surgery and Interventional Science (Division of) > Institute of Orthopaedics and Musculoskeletal Science

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