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Detection of vimentin-specific autoreactive CD8+ T cells in cardiac transplant patients

Barber, L; Whitelegg, A; Madrigal, J; Banner, N; Rose, M; (2004) Detection of vimentin-specific autoreactive CD8+ T cells in cardiac transplant patients. TRANSPLANTATION , 77 (10) pp. 1604-1609.

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Abstract

BACKGROUND: Evidence is emerging that autoimmunity can play a role in allograft rejection. Reports have described the presence of autoantibodies in transplant patients and CD4+ autoreactive T cells in rodent models of allograft rejection. The objective of this study was to seek evidence of CD8+ T-cell-mediated autoimmunity in the transplant setting. The author have previously observed autoimmunity to the non-polymorphic cytoskeletal protein vimentin in cardia transplant patients. In this study, vimentin antibody positive patients were screened for the presence of vimentin-specific self- major histocompatibility complex class I-restricted CD8+ T cells. METHODS: Two peptide sequences from vimentin that bound HLA-A*0201 were identified and fluorochrome-labeled A*0201 tetramers with each peptide were constructed to screen for vimentin-specific T cells. RESULTS: Tetramer-binding CD8+ T cells were detected in peripheral blood lymphocytes from two of six patients after expansion by in vitro stimulation with peptide. Tetramer-binding T cells produced interferon-gamma in an antigen-specific fashion. No autoreactive T cells specific for vimentin were detected after peptide stimulation of T cells from eight healthy A*0201-positive volunteers. CONCLUSIONS: This finding is the first evidence of CD8+ T-cell-mediated autoimmunity in human transplant patients

Type: Article
Title: Detection of vimentin-specific autoreactive CD8+ T cells in cardiac transplant patients
Additional information: Language: EnglishSubset: IMJournal code: 0000132144Document status: MedlinePublication model: PrintDataStar source field: Transplantation, {Transplantation}, 27 May 2004, vol. 77, no. 10, p. 1604-9, ISSN: 0041-1337DataStar update date: 20050101
Keywords: 0 (Autoantibodies), 0 (HLA-A*0201-antigen), 0 (HLA-A-Antigens), 0 (Peptide-Fragments), 0 (Vimentin), 82115-62-6 (Interferon-Type-II), ADULT, allograft, antibody, ANTIBODY-SPECIFICITY/*, AUTOANTIBODIES/*IM (immunology), autoimmunity, CASE-CONTROL-STUDIES, CD8-POSITIVE-T-LYMPHOCYTES/*IM (immunology), ME (metabolism), FEMALE, HEART-TRANSPLANTATION/*IM (immunology), histocompatibility-complex, HLA-A-ANTIGENS/AN (analysis), CH (chemistry), IM (immunology), hospital, human, HUMANS, IN-VITRO, INTERFERON-TYPE-II/BI (biosynthesis), language, LONDON, lymphocyte, major-histocompatibility-complex, MALE, MEDLINE, MIDDLE-AGED, model, patient, peptide, PEPTIDE-FRAGMENTS/IM (immunology), protein, publication, RESEARCH-SUPPORT-NON-U-S-GOVT, rodent, tetramer, TRANSPLANTATION, United-Kingdom, VIMENTIN/*IM (immunology)
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Haematology
URI: http://discovery.ucl.ac.uk/id/eprint/181904
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