Moesin controls cortical rigidity, cell rounding, and spindle morphogenesis during mitosis.
91 - 101.
Background: During mitosis, animal cells undergo a complex sequence of morphological changes, from retraction of the cell margin and cell rounding at the onset of mitosis to axial elongation and cytokinesis at mitotic exit. The molecular mechanisms driving the early changes in mitotic cell form and their functional significance, however, remain unknown. Here we identify Moesin as a key player. Moesin is the sole Drosophila member of the ERM proteins, which, once activated via phosphorylation, crosslink actin filaments to the cytoplasmic tails of plasma membrane proteins.Results: We find that the Moesin is activated upon entry into mitosis, is necessary for the accompanying increase in cortical rigidity and cell rounding and, when artificially activated, is sufficient to induce both processes in interphase cells, independently of Myosin II. This phospho-Moesin-induced increase in cortical rigidity plays an important role during mitotic progression, because spindle morphogenesis and chromosome alignment are compromised in Moesin RNAi cells. Significantly, however, the spindle defects observed in soft metaphase cells can be rescued by the re-establishment of cortical tension from outside the cell.Conclusions: These data show that changes in the activity and localization of Moesin that accompany mitotic progression contribute to the establishment of a stiff, rounded cortex at metaphase and to polar relaxation at anaphase and reveal the importance of this Moesin-induced increase in cortical rigidity for spindle morphogenesis and orderly chromosome segregation. In doing so, they help to explain why dynamic changes in cortical architecture are a universal feature of mitosis in animal cells.
|Title:||Moesin controls cortical rigidity, cell rounding, and spindle morphogenesis during mitosis|
|Keywords:||ATOMIC-FORCE MICROSCOPY, ERM PROTEIN MOESIN, MITOTIC SPINDLE, EZRIN/RADIXIN/MOESIN PROTEINS, ACTIN-BINDING, MOLECULAR REQUIREMENTS, EPITHELIAL INTEGRITY, LIGHT-CHAIN, S2 CELLS, CYTOKINESIS|
|UCL classification:||UCL > School of BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology|
Archive Staff Only