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LTP but not seizure is associated with up-regulation of AKAP-150

Genin, A; French, P; Doyere, V; Davis, S; Errington, ML; Maroun, M; Stean, T; ... O'Connor, V; + view all (2003) LTP but not seizure is associated with up-regulation of AKAP-150. EUR J NEUROSCI , 17 (2) 331 - 340. 10.1046/j.1460-9568.2003.02462.x.

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Abstract

We have used differential display to profile and compare the mRNAs expressed in the hippocampus of freely moving animals after the induction of long-term potentiation (LTP) at the perforant path-dentate gyrus synapse with control rats receiving low-frequency stimulation. We have combined this with in situ hybridization and have identified A-kinase anchoring protein of 150 kDa (AKAP-150 ) as a gene selectively up-regulated during the maintenance phase of LTP. AKAP-150 mRNA has a biphasic modulation in the dentate gyrus following the induction of LTP. The expression of AKAP-150 was 29% lower than stimulated controls 1 h after the induction of LTP. Its expression was enhanced 3 (50%), 6 (239%) and 12 h (210%) after induction, returning to control levels by 24 h postinduction. The NMDA receptor antagonist CPP blocked the tetanus-induced modulation of AKAP-150 expression. Interestingly, strong generalized stimulation produced by electroconvulsive shock did not increase the expression of AKAP-150 . This implies that the AKAP-150 harbours a novel property of selective responsiveness to the stimulation patterns that trigger NMDA-dependent LTP in vivo . Its selective up-regulation during LTP and its identified functions as a scaffold for protein kinase A, protein kinase C, calmodulin, calcineurin and ionotropic glutamate receptors suggest that AKAP-150 encodes is an important effector protein in the expression of late LTP.

Type: Article
Title: LTP but not seizure is associated with up-regulation of AKAP-150
DOI: 10.1046/j.1460-9568.2003.02462.x
Keywords: differential display, ECS, gene expression, hippocampus, rat, synaptic plasticity, LONG-TERM POTENTIATION, IMMEDIATE-EARLY GENE, PROTEIN-KINASE-A, ELEMENT-BINDING PROTEIN, DENTATE GYRUS, MESSENGER-RNA, SYNAPTIC ACTIVITY, LATE-PHASE, IN-VIVO, GLUTAMATE RECEPTORS
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: http://discovery.ucl.ac.uk/id/eprint/179189
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