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Investigation of the self-association and hetero-association interactions of H-NS and StpA from Enterobacteria

Leonard, PG; Ono, S; Gor, J; Perkins, SJ; Ladbury, JE; (2009) Investigation of the self-association and hetero-association interactions of H-NS and StpA from Enterobacteria. MOL MICROBIOL , 73 (2) 165 - 179. 10.1111/j.1365-2958.2009.06754.x.

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Abstract

The nucleoid-associated protein H-NS and its paralogue StpA are global regulators of gene expression and form an integral part of the protein scaffold responsible for DNA condensation in Escherichia coli and Salmonella typhimurium. Although protein oligomerization is a requirement for this function, it is not entirely understood how this is accomplished. We address this by reporting on the self-association of H-NS and its hetero-association with StpA. We identify residues 1-77 of H-NS as being necessary and sufficient for high-order association. A multitechnique-based approach was used to measure the effects of salt concentration on the size distribution of H-NS and the thermal stability of H-NS and StpA dimers. The thermal stability of the StpA homodimer is significantly greater than that of H-NS1-74. Investigation of the hetero-association of H-NS and StpA proteins suggested that the association of H-NS with StpA is more stable than the self-association of either H-NS or StpA with themselves. This provides a clear understanding of the method of oligomerization of these important proteins in effecting DNA condensation and reveals that the different associative properties of H-NS and StpA allow them to perform distinct, yet complementary roles in the bacterial nucleoid.

Type: Article
Title: Investigation of the self-association and hetero-association interactions of H-NS and StpA from Enterobacteria
DOI: 10.1111/j.1365-2958.2009.06754.x
Keywords: SECONDARY STRUCTURE PREDICTION, NUCLEOID-ASSOCIATED PROTEIN, CHROMATIN-STRUCTURING PROTEIN, ENTERICA SEROVAR TYPHIMURIUM, ESCHERICHIA-COLI, DIMERIZATION DOMAIN, REGULATORY PROTEIN, GENE-EXPRESSION, OLIGOMERIZATION DOMAIN, MOLECULAR ADAPTER
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/178026
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