Nibali, L and Tonetti, MS and Ready, D and Parkar, M and Brett, PM and Donos, N and D'Aiuto, F (2008) Interleukin-6 polymorphisms are associated with pathogenic bacteria in subjects with periodontitis. J PERIODONTOL , 79 (4) 677 - 683. 10.1902/jop.2008.070453.
Full text not available from this repository.
Background: Growing evidence suggests that individual genetic susceptibility may influence the host's response to infections. Previously, we showed that a common variation in the interleukin (IL)-6 gene was associated with increased odds of detection of common periodontal pathogens from individuals with aggressive periodontitis. The aim of this study was to investigate the association between IL-6 polymorphisms and periodontopathogenic bacteria in a larger, ethnically mixed population of subjects with periodontitis.Methods: Genomic DNA was extracted from 107 subjects diagnosed with severe forms of periodontitis to study a cluster of polymorphisms in inflammatory genes, including IL-6. The presence of Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans), Porphyromonas gingivalis, and Tannerella forsythia (previously T forsythensis) in their subgingival biofilm was determined by polymerase chain reaction analysis. Serum IL-6 and C-reactive protein (CRP) concentrations were analyzed by enzyme-linked immunosorbent assay.Results: Multiple logistic regression analysis revealed that the IL-6 -6106 polymorphism was associated with the detection of A. actinomycetemcomitans (P= 0.009; odds ratio [OR] = 3.5; 95% confidence interval [Cl]: 1.38 to 9.16) and the concomitant detection of A. actinomycetemcomitans and P. gingivalis (P = 0.015; OR = 3.6; 95% Cl: 1.28 to 10.04). The IL-6-174 polymorphism was associated with increased odds of the concomitant detection of A. actinomycetemcomitans and P. gingivalis (P= 0.042; OR = 2.8; 95% Cl: 1.04 to 7.75). Haplotype analysis of all five IL-6 polymorphisms confirmed an association with the detection of A. actinomycetemcomitans (P= 0.046). The IL-6 -6106 polymorphism was also associated with CRP serum levels at multivariate analysis (P= 0.024).Conclusions: These findings confirm the hypothesis that complex interactions between the microbiota and host genome are at the basis of susceptibility to periodontitis. Periodontal disease may represent a useful model to study the pathways and mechanisms of host-pathogen interactions in inflammatory diseases.
|Title:||Interleukin-6 polymorphisms are associated with pathogenic bacteria in subjects with periodontitis|
|Keywords:||bacteria, genetic polymorphisms, interleukin-6, periodontitis, GENE POLYMORPHISMS, ACTINOBACILLUS-ACTINOMYCETEMCOMITANS, SYSTEMIC INFLAMMATION, RISK-FACTORS, IL-6 GENE, DISEASE, SUSCEPTIBILITY, INFECTION, GINGIVALIS, PREVALENCE|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Biomaterials and Tissue Engineering|
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Clinical Research
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Restorative Dental Sciences
Archive Staff Only: edit this record