Human melanomas express functional P2X(7) receptors.
CELL TISSUE RES
411 - 418.
Adenosine 5'-triphosphate is known to function as a potent extracellular messenger, producing its effects via a distinct family of cell surface receptors. Different receptor subtypes have been shown to modulate different cellular functions such as proliferation, differentiation and apoptosis. We have investigated the functional expression and apoptotic action of the P2X(7) receptor in human malignant melanoma tissue and cells. Incubation of cells with the potent P2X(7) receptor agonist 2'-3'-O-(4-benzoyl-benzoyl) adenosine 5'-triphosphate leads to a decrease in cell number, which is dose-dependent and reversible by the antagonist 1-N,O-bis-[5-isoquinoline-sulfonyl]-N-methyl-L-tyrosyl)-4-phenyl-piperazine. Synthesis of the P2X(7) receptor by these cells has been established by reverse transcriptase-polymerase chain reaction, immunohistochemistry, immunocytochemistry and cellular accumulation of the fluorescent DNA-binding dye YO-PRO-1. The P2X(7) receptors have been shown to mediate apoptotic actions of extracellular nucleotides and represent a novel target for melanoma therapy.
|Title:||Human melanomas express functional P2X(7) receptors|
|Keywords:||purinergic receptors, ATP, P2X(7), melanoma, cancer, human, HUMAN-MALIGNANT MELANOMA, EXTRACELLULAR ATP, PURINERGIC RECEPTORS, ADENOSINE RECEPTORS, TUMOR-CELLS, NUCLEOTIDE RECEPTOR, DENDRITIC CELLS, CALCIUM INFLUX, CANCER-CELLS, DIFFERENTIATION|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of)
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Surgery and Interventional Science (Division of)
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