UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

NMR structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system

Bayliss, R; Harris, R; Coutte, L; Monier, A; Fronzes, R; Christie, PJ; Driscoll, PC; (2007) NMR structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system. P NATL ACAD SCI USA , 104 (5) 1673 - 1678. 10.1073/pnas.0609535104. Gold open access

Abstract

Type IV secretion (T4S) systems translocate DNA and protein effectors through the double membrane of Gram-negative bacteria. The paradigmatic T4S system in Agrobacterium tumetaciens is assembled from 11 VirB subunits and VirD4. Two subunits, VirB9 and VirB7, form an important stabilizing complex in the outer membrane. We describe here the NMR structure of a complex between the C-terminal domain of the VirB9 homolog TraO (TraO(CT)), bound to VirB7-like TraN from plasmid pKM101. TraO(CT) forms a beta-sandwich around which TraN winds. Structure-based mutations in VirB7 and Vir!39 of A. tumefaciens show that the heterodimer interface is conserved. Opposite this interface, the TraO structure shows a protruding three-stranded beta-appendage, and here, we supply evidence that the corresponding region of VirB9 of A. tumefaciens inserts in the membrane and protrudes extracellularly. This complex structure elucidates the molecular basis for the interaction between two essential components of a T4S system.

Type: Article
Title: NMR structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system
Open access status: An open access publication
DOI: 10.1073/pnas.0609535104
Publisher version: http://www.ncbi.nlm.nih.gov/pmc/ articles/PMC17852...
Keywords: TraO-TraN, structural biology, bacterial conjugation, pilus, DNA transfer, AGROBACTERIUM-TUMEFACIENS VIRB7, TRANSPORT APPARATUS, PROTEINS, DNA, TOPOLOGY, ASSOCIATION, LIPOPROTEIN, CONJUGATION, BIOGENESIS
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/176672
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item