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Scanning elastic scattering spectroscopy detects metastatic breast cancer in sentinel lymph nodes

Austwick, MR; Clark, B; Mosse, CA; Johnson, K; Chicken, DW; Somasundaram, SK; ... Keshtgar, MRS; + view all (2010) Scanning elastic scattering spectroscopy detects metastatic breast cancer in sentinel lymph nodes. J BIOMED OPT , 15 (4) , Article 047001. 10.1117/1.3463005. Gold open access

Abstract

A novel method for rapidly detecting metastatic breast cancer within excised sentinel lymph node(s) of the axilla is presented. Elastic scattering spectroscopy (ESS) is a point-contact technique that collects broadband optical spectra sensitive to absorption and scattering within the tissue. A statistical discrimination algorithm was generated from a training set of nearly 3000 clinical spectra and used to test clinical spectra collected from an independent set of nodes. Freshly excised nodes were bivalved and mounted under a fiber-optic plate. Stepper motors raster-scanned a fiber-optic probe over the plate to interrogate the node's cut surface, creating a 20 x 20 grid of spectra. These spectra were analyzed to create a map of cancer risk across the node surface. Rules were developed to convert these maps to a prediction for the presence of cancer in the node. Using these analyses, a leave-one-out cross-validation to optimize discrimination parameters on 128 scanned nodes gave a sensitivity of 69% for detection of clinically relevant metastases (71% for macrometastases) and a specificity of 96%, comparable to literature results for touch imprint cytology, a standard technique for intra-operative diagnosis. ESS has the advantage of not requiring a pathologist to review the tissue sample. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3463005]

Type:Article
Title:Scanning elastic scattering spectroscopy detects metastatic breast cancer in sentinel lymph nodes
Open access status:An open access publication
DOI:10.1117/1.3463005
Publisher version:http://www.ncbi.nlm.nih.gov/pmc/ articles/PMC2917446/?tool=pubmed
Keywords:elastic scattering spectroscopy, backscattering, breast cancer, sentinel lymph node biopsy, IMPRINT CYTOLOGY, BIOPSY, REFLECTANCE, VALIDATION, DYSPLASIA
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Surgery and Interventional Science (Division of) > Research Department of General Surgery
UCL > School of BEAMS > Faculty of Maths and Physical Sciences > Statistical Science
UCL > School of BEAMS > Faculty of the Built Environment > Centre for Advanced Spatial Analysis

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