Zolfaghari, PS;
Packer, S;
Singer, M;
Nair, SP;
Bennett, J;
Street, C;
Wilson, M;
(2009)
In vivo killing of Staphylococcus aureus using a light-activated antimicrobial agent.
BMC MICROBIOL
, 9
, Article 27. 10.1186/1471-2180-9-27.
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Abstract
Background: The widespread problem of antibiotic resistance in pathogens such as Staphylococcus aureus has prompted the search for new antimicrobial approaches. In this study we report for the first time the use of a light-activated antimicrobial agent, methylene blue, to kill an epidemic methicillin-resistant Staphylococcus aureus (EMRSA-16) strain in two mouse wound models.Results: Following irradiation of wounds with 360 J/cm(2) of laser light (670 nm) in the presence of 100 mu g/ml of methylene blue, a 25-fold reduction in the number of viable EMRSA was seen. This was independent of the increase in temperature of the wounds associated with the treatment. Histological examination of the wounds revealed no difference between the photodynamic therapy (PDT)-treated wounds and the untreated wounds, all of which showed the same degree of inflammatory infiltration at 24 hours.Conclusion: The results of this study demonstrate that PDT is effective at reducing the total number of viable EMRSA in a wound. This approach has promise as a means of treating wound infections caused by antibiotic-resistant microbes as well as for the elimination of such organisms from carriage sites.
| Type: | Article |
|---|---|
| Title: | In vivo killing of Staphylococcus aureus using a light-activated antimicrobial agent |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1186/1471-2180-9-27 |
| Publisher version: | http://www.biomedcentral.com/1471-2180/9/27 |
| Language: | English |
| Additional information: | © 2009 Zolfaghari et al; licensee BioMed Central Ltd.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | TARGETED PHOTODYNAMIC THERAPY, WOUND INFECTIONS, TOLUIDINE BLUE, INACTIVATION, RESISTANCE, BIOFILMS, BACTERIA, MICE, MRSA |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Experimental and Translational Medicine UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Microbial Diseases |
| URI: | https://discovery.ucl.ac.uk/id/eprint/174861 |
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