UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Variable DNA methylation of transposable elements: The case study of mouse Early Transposons

Reiss, D; Zhang, Y; Rouhi, A; Reuter, M; Mager, DL; (2010) Variable DNA methylation of transposable elements: The case study of mouse Early Transposons. Epigenetics , 5 (1) 68 - 79. 10.4161/epi.5.1.10631. Green open access

[thumbnail of Reuter_epi%2E5%2E1%2E10631.pdf]
Preview
Text
Reuter_epi%2E5%2E1%2E10631.pdf

Download (1MB) | Preview

Abstract

Phenotypic variation stems from both genetic and epigenetic differences between individuals. In order to elucidate how phenotypes are determined, it is necessary to understand the forces that generate variation in genome sequence as well as its epigenetic state. In both contexts, transposable elements (TEs) may play an important role. It is well established that TE activity is a major generator of genetic variation, but recent research also suggests that TEs contribute to epigenetic variation. Stochastic epigenetic silencing of some TE insertions in mice has been shown to cause phenotypic variability between individuals. However, the prevalence of this phenomenon has never been evaluated. Here, we use 18 insertions of a mouse Endogenous Retrovirus (ERV) family, the Early Transposons (ETns), to detect insertion-dependent determinants of DNA methylation levels and variability between both cells and individuals. We show that the structure and age of insertions influence methylation levels and variability, resulting in a subgroup of loci that displays unexpectedly high variability in methylation and suggesting stochastic events during methylation establishment. Despite variation in methylation according to the age and structure of each locus, homologous CpG sites show similar tendencies in methylation levels across loci, emphasizing the role of the insertion's sequence in methylation determination. Our results show that differences in methylation of ETns between individuals is not a sporadic phenomenon and support the hypothesis that ERVs contribute to phenotypic variability through their stochastic silencing.

Type: Article
Title: Variable DNA methylation of transposable elements: The case study of mouse Early Transposons
Open access status: An open access version is available from UCL Discovery
DOI: 10.4161/epi.5.1.10631
Publisher version: http://dx.doi.org/10.4161/epi.5.1.10631
Language: English
Additional information: This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License (https://creativecommons.org/licenses/by-nc/3.0/). The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
Keywords: inter-individual, variability, DNA methylation, retrotransposons, mouse, long terminal repeats, LONG TERMINAL REPEAT, APRT GENE PROMOTER, EPIGENETIC INHERITANCE, MOLECULAR-BASIS, AGOUTI LOCUS, EXPRESSION, RETROTRANSPOSON, SEQUENCES, GENOME, DROSOPHILA
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment
URI: https://discovery.ucl.ac.uk/id/eprint/170571
Downloads since deposit
97Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item