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The role of V delta 2-negative gamma delta T cells during cytomegalovirus reactivation in recipients of allogeneic stem cell transplantation

Knight, A; Madrigal, AJ; Grace, S; Sivakumaran, J; Kottaridis, P; Mackinnon, S; Travers, PJ; (2010) The role of V delta 2-negative gamma delta T cells during cytomegalovirus reactivation in recipients of allogeneic stem cell transplantation. BLOOD , 116 (12) 2164 - 2172. 10.1182/blood-2010-01-255166.

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Abstract

Reactivation of cytomegalovirus (CMV) remains a serious complication after allogeneic stem cell transplantation, but the role of gamma delta T cells is undefined. We have studied the immune reconstitution of V delta 2negative (V delta 2neg) gamma delta T cells, including V delta 1 and V delta 3 subsets and V delta 2positive (V delta 2pos) gamma delta T cells in 40 patients during the first 24 months after stem cell transplantation. Significant long-term expansions of V delta 2neg but not V delta 2pos gamma delta T cells were observed during CMV reactivation early after transplantation, suggesting direct involvement of gamma delta T cells in anti-CMV immune responses. Similarly, significantly higher numbers of V delta 2neg gamma delta T cells were detected in CMV-seropositive healthy persons compared with seronegative donors; the absolute numbers of V delta 2pos cells were not significantly different. The expansion of V delta 2neg gamma delta T cells appeared to be CMV-related because it was absent in CMV-negative/ Epstein-Barr virus-positive patients. T cell receptor-delta chain determining region 3 spectratyping of V delta 2neg gamma delta T cells in healthy subjects and patients showed restricted clonality. Polyclonal V delta 2neg cell lines generated from CMV-seropositive healthy donors and from a recipient of a graft from a CMV-positive donor lysed CMV-infected targets in all cases. Our study shows new evidence for role of gamma delta T cells in the immune response to CMV reactivation in transplantation recipients. (Blood. 2010;116(12):2164-2172)

Type: Article
Title: The role of V delta 2-negative gamma delta T cells during cytomegalovirus reactivation in recipients of allogeneic stem cell transplantation
DOI: 10.1182/blood-2010-01-255166
Keywords: RECEPTOR REPERTOIRE, PERIPHERAL-BLOOD, INFECTED PERSONS, IMMUNE-RESPONSES, NKG2D RECEPTOR, CMV DISEASE, BONE-MARROW, B-CELLS, LYMPHOCYTES, V-DELTA-1
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Haematology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
URI: http://discovery.ucl.ac.uk/id/eprint/168085
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