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Clinical evidence of efficient tumor targeting based on single-chain Fv antibody selected from a combinatorial library

Begent, RHJ; Verhaar, MJ; Chester, KA; Casey, JL; Green, AJ; Napier, MP; Hopstone, LD; ... Robson, L; + view all (1996) Clinical evidence of efficient tumor targeting based on single-chain Fv antibody selected from a combinatorial library. Nature Medicine , 2 (9) pp. 979-984.

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Abstract

We present a system for cancer targeting based on single-chain Fv (scFv) antibodies selected from combinatorial libraries, produced in bacteria and purified by using an engineered tag. Combinatorial libraries of scFv genes contain great diversity, and scFv antibodies with characteristics optimized for a particular task can be selected from them using filamentous bacteriophage. We illustrate the benefits of this system by imaging patients with carcinoembryonic antigen (CEA)-producing cancers using an iodine-123 labeled scFv anti-CEA selected for high affinity. All known tumor deposits were located, and advantages over current imaging technology are illustrated. ScFvs are produced in a cloned form and can be readily engineered to have localizing and therapeutic functions that will be applicable in cancer and other diseases

Type: Article
Title: Clinical evidence of efficient tumor targeting based on single-chain Fv antibody selected from a combinatorial library
Keywords: CARCINOEMBRYONIC ANTIGEN; RADIOLABELED ANTIBODY; AFFINITY MATURATION; COLORECTAL-CANCER; PHAGE ANTIBODIES; GENERATION; THERAPY; CONSTRUCTION; PROTEIN
UCL classification: UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Wolfson Institute and Cancer Institute Administration > Cancer Institute
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Wolfson Institute and Cancer Institute Administration > Cancer Institute > Research Department of Cancer Biology
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Wolfson Institute and Cancer Institute Administration > Cancer Institute > Research Department of Oncology
URI: http://discovery.ucl.ac.uk/id/eprint/165877
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