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alpha,alpha '-Dihydroxy Ketones and 2-Amino-1,3-diols: Synthetic and Process Strategies Using Biocatalysts

Hailes, HC; Dalby, PA; Lye, GJ; Baganz, F; Micheletti, M; Szita, N; Ward, JM; (2010) alpha,alpha '-Dihydroxy Ketones and 2-Amino-1,3-diols: Synthetic and Process Strategies Using Biocatalysts. CURR ORG CHEM , 14 (17) 1883 - 1893.

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Abstract

There is increasing interest in the use of biocatalysts in synthetic applications due to their ability to achieve highly stereoselective and atom efficient conversions. Recent developments in molecular biology techniques and synthetic biology have also enhanced potential applications using non-natural substrates. Here we review strategies to alpha,alpha'-dihydroxy ketones and 2-amino-1,3-diols via the use of transketolase, a carbon-carbon bond forming biocatalyst, and the enzyme transaminase which converts aldehydes and ketones to amines. Using an integrated strategy we have investigated new chemistries and assays, identified novel biocatalysts and used directed evolution strategies, together with miniaturization studies and modelling to achieve rapid and predictive process scale-up.

Type:Article
Title:alpha,alpha '-Dihydroxy Ketones and 2-Amino-1,3-diols: Synthetic and Process Strategies Using Biocatalysts
Keywords:Biocatalysis, transketolase, transaminase, dihydroxy ketones, 2-amino-1,3-diols, microwell studies, microfluidics, colorimetric assay, ESCHERICHIA-COLI TRANSKETOLASE, DIRECTED EVOLUTION, BOND FORMATION, SUBSTRATE-SPECIFICITY, MICROTITRE PLATES, PYRUVATE TRANSAMINASE, MICROFLUIDIC DEVICES, YEAST TRANSKETOLASE, EXPERIMENTAL-DESIGN, CATALYZED SYNTHESIS
UCL classification:UCL > School of BEAMS > Faculty of Engineering Science > Biochemical Engineering
UCL > School of BEAMS > Faculty of Maths and Physical Sciences > Chemistry

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