UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Variation within Mycobacterium scrofulaceum

Khosravi, AD; Stanford, JL; Donoghue, HD; Rook, GAW; (1997) Variation within Mycobacterium scrofulaceum. J APPL MICROBIOL , 83 (5) 596 - 602.

Full text not available from this repository.

Abstract

The purpose of this study was to identify Mycobacterium scrofulaceum reliably and rapidly and investigate diversity within the species. Fifty-four cultures were identified as Myco. scrofulaceum by preliminary cultural and biochemical tests, thin-layer chromatography and double diffusion. These strains were examined by PCR based on the 65 kDa heat stress protein gene, followed by restriction enzyme analysis of the product with BstEII and HaeIII. This produced seven groups, most with fewer fragments than had been reported previously. The technique was a rapid and reliable method for studying variation within Myco. scrofulaceum but alone, was unable to discriminate between some of these variants and other genetically similar species. When PCR-RFLP results were combined with biochemical tests, the major groups appeared to relate to different disease situations and thus, may have some epidemiological value.

Type:Article
Title:Variation within Mycobacterium scrofulaceum
Keywords:POLYMERASE CHAIN-REACTION, GROWING MYCOBACTERIA, IDENTIFICATION, AMPLIFICATION, TUBERCULOSIS, TAXONOMY, PROTEIN
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Infection and Immunity (Division of) > Research Department of Infection

Archive Staff Only: edit this record