Rama, S;
(2015)
Shift and Mean Algorithm for Functional Imaging with High Spatio-Temporal Resolution.
Frontiers in Cellular Neuroscience
, 9
, Article 446. 10.3389/fncel.2015.00446.
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Abstract
Understanding neuronal physiology requires to record electrical activity in many small and remote compartments such as dendrites, axon or dendritic spines. To do so, electrophysiology has long been the tool of choice, as it allows recording very subtle and fast changes in electrical activity. However, electrophysiological measurements are mostly limited to large neuronal compartments such as the neuronal soma. To overcome these limitations, optical methods have been developed, allowing the monitoring of changes in fluorescence of fluorescent reporter dyes inserted into the neuron, with a spatial resolution theoretically only limited by the dye wavelength and optical devices. However, the temporal and spatial resolutive power of functional fluorescence imaging of live neurons is often limited by a necessary trade-off between image resolution, signal to noise ratio (SNR) and speed of acquisition. Here, I propose to use a Super-Resolution Shift and Mean (S&M) algorithm previously used in image computing to improve the SNR, time sampling and spatial resolution of acquired fluorescent signals. I demonstrate the benefits of this methodology using two examples: voltage imaging of action potentials (APs) in soma and dendrites of CA3 pyramidal cells and calcium imaging in the dendritic shaft and spines of CA3 pyramidal cells. I show that this algorithm allows the recording of a broad area at low speed in order to achieve a high SNR, and then pick the signal in any small compartment and resample it at high speed. This method allows preserving both the SNR and the temporal resolution of the signal, while acquiring the original images at high spatial resolution.
| Type: | Article |
|---|---|
| Title: | Shift and Mean Algorithm for Functional Imaging with High Spatio-Temporal Resolution |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3389/fncel.2015.00446 |
| Publisher version: | https://doi.org/10.3389/fncel.2015.00446 |
| Language: | English |
| Additional information: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Experimental Epilepsy |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1575779 |
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