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Assessing the effects of mitofusin 2 deficiency in the adult heart using 3D electron tomography

Beikoghli Kalkhoran, S; Hall, AR; White, IJ; Cooper, J; Fan, Q; Ong, S-B; Hernández-Reséndiz, S; ... Hausenloy, DJ; + view all (2017) Assessing the effects of mitofusin 2 deficiency in the adult heart using 3D electron tomography. Physiological Reports , 5 (17) 10.14814/phy2.13437. Green open access

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Abstract

The effects of mitofusin 2 (MFN2) deficiency, on mitochondrial morphology and the mitochondria-junctional sarcoplasmic reticulum (jSR) complex in the adult heart, have been previously investigated using 2D electron microscopy, an approach which is unable to provide a 3D spatial assessment of these imaging parameters. Here, we use 3D electron tomography to show that MFN2-deficient mitochondria are larger in volume, more elongated, and less rounded; have fewer mitochondria-jSR contacts, and an increase in the distance between mitochondria and jSR, when compared to WT mitochondria. In comparison to 2D electron microscopy, 3D electron tomography can provide further insights into mitochondrial morphology and the mitochondria-jSR complex in the adult heart.

Type: Article
Title: Assessing the effects of mitofusin 2 deficiency in the adult heart using 3D electron tomography
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.14814/phy2.13437
Publisher version: http://dx.doi.org/10.14814/phy2.13437
Language: English
Additional information: © 2017 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Keywords: 3D electron tomography, MFN2 KO, mitochondria–jSR junction
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > Institute of Cardiovascular Science
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > Institute of Cardiovascular Science > Pre-clinical and Fundamental Science
URI: http://discovery.ucl.ac.uk/id/eprint/1574534
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