UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

The Effect of Hypothermic and Cryogenic Preservation on Engineered Neural Tissue

Day, AGE; Bhangra, KS; Murray-Dunning, C; Stevanato, L; Phillips, J; (2017) The Effect of Hypothermic and Cryogenic Preservation on Engineered Neural Tissue. Tissue Engineering Part C: Methods , 23 (10) pp. 575-582. 10.1089/ten.TEC.2017.0244. Green open access

[img]
Preview
Text
ten.tec.2017.0244.pdf - ["content_typename_Published version" not defined]

Download (592kB) | Preview

Abstract

This study explored different approaches to preserve Engineered Neural Tissue (EngNT), a stabilised cellular collagen hydrogel containing columns of aligned Schwann cells for nervous system repair. The ability to preserve EngNT without disrupting cellular and extracellular components and structures is important for clinical translation and commercialisation. Stabilised cellular gels and EngNT constructs were preserved under various conditions and cell survival assessed using live/dead microscopy and metabolic assay. Optimal survival was recorded in hypothermic (4ºC) conditions for 2-3 days using Hibernate®-A media, and, for longer term cryogenic storage (liquid nitrogen), using a mixture of 60% Dulbecco's Modified Eagle's Medium media (DMEM), 30% foetal calf serum (FCS) and 10% dimethyl sulfoxide (DMSO). Functionality and structure of preserved EngNT was assessed in co-culture with dorsal root ganglion (DRG) neurons, which indicated that alignment of Schwann cells and the ability of EngNT to support and guide neuronal regeneration were not disrupted. The identification of conditions that preserve EngNT will inform development of storage and transport methodologies to support clinical and commercial translation of this technology and other therapies based on cellular hydrogels.

Type: Article
Title: The Effect of Hypothermic and Cryogenic Preservation on Engineered Neural Tissue
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1089/ten.TEC.2017.0244
Publisher version: http://dx.doi.org/10.1089/ten.TEC.2017.0244
Language: English
Additional information: © Adam G.E. Day et al. 2017; Published by Mary Ann Liebert, Inc. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: Collagen, Schwann cell, preservation, hypothermic, cryogenic, nerve guide, nerve regeneration, nerve tissue engineering, hydrogel
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmacology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute
URI: http://discovery.ucl.ac.uk/id/eprint/1574488
Downloads since deposit
36Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item