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Cryopreservation and re-culture of a 2.3 litre biomass for use in a bioartificial liver device

Kilbride, P; Lamb, S; Gibbons, S; Bundy, J; Erro, E; Selden, C; Fuller, B; (2017) Cryopreservation and re-culture of a 2.3 litre biomass for use in a bioartificial liver device. PLoS One , 12 (8) , Article e0183385. 10.1371/journal.pone.0183385. Green open access

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Abstract

For large and complex tissue engineered constructs to be available on demand, long term storage using methods, such as cryopreservation, are essential. This study optimised parameters such as excess media concentration and warming rates and used the findings to enable the successful cryopreservation of 2.3 litres of alginate encapsulated liver cell spheroids. This volume of biomass is typical of those required for successful treatment of Acute Liver Failure using our Bioartificial Liver Device. Adding a buffer of medium above the biomass, as well as slow (0.6°C/min) warming rates was found to give the best results, so long as the warming through the equilibrium melting temperature was rapid. After 72 h post thaw-culture, viable cell number, glucose consumption, lactate production, and alpha-fetoprotein production had recovered to pre-freeze values in the 2.3 litre biomass (1.00 ± 0.05, 1.19 ± 0.10, 1.23 ± 0.18, 2.03 ± 0.04 per ml biomass of the pre-cryopreservation values respectively). It was also shown that further improvements in warming rates of the biomass could reduce recovery time to < 48 h. This is the first example of a biomass of this volume being successfully cryopreserved in a single cassette and re-cultured. It demonstrates that a bioartificial liver device can be cryopreserved, and has wider applications to scale-up large volume cryopreservation.

Type: Article
Title: Cryopreservation and re-culture of a 2.3 litre biomass for use in a bioartificial liver device
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1371/journal.pone.0183385
Publisher version: https://doi.org/10.1371/journal. pone.0183385
Language: English
Additional information: © 2017 Kilbride et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
UCL classification: UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences
URI: http://discovery.ucl.ac.uk/id/eprint/1573283
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