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Comparative analysis of LytS/LytTR-type histidine kinase/response regulator systems in γ-proteobacteria

Behr, S; Brameyer, S; Witting, M; Schmitt-Kopplin, P; Jung, K; (2017) Comparative analysis of LytS/LytTR-type histidine kinase/response regulator systems in γ-proteobacteria. PLoS ONE , 12 (8) , Article e0182993. 10.1371/journal.pone.0182993. Green open access

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Abstract

Bacterial histidine kinase/response regulator systems operate at the interface between environmental cues and physiological states. Escherichia coli contains two LytS/LytTR-type histidine kinase/response regulator systems, BtsS/BtsR (formerly YehU/YehT) and YpdA/YpdB, which have been identified as pyruvate-responsive two-component systems. Since they exhibit remarkable similarity, we analyzed their phylogenetic distribution within the γ-proteobacteria, and experimentally characterized them in a set of representative species. We found that BtsS/BtsR is the predominant LytS/LytTR-type two-component system among γ-proteobacteria, whereas YpdA/YpdB primarily appears in a supplementary role. Based on our observations in E. coli, we used the highly conserved DNA-binding motifs to test the in vivo functionality of both systems in various genera, including Salmonella, Enterobacter, Citrobacter, Xenorhabdus, Yersinia, Aeromonas and Vibrio. The results suggest that, in all cases tested, BtsS/BtsR and YpdA/YpdB respond to different levels of pyruvate in the environment.

Type: Article
Title: Comparative analysis of LytS/LytTR-type histidine kinase/response regulator systems in γ-proteobacteria
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1371/journal.pone.0182993
Publisher version: https://doi.org/10.1371/journal.pone.0182993
Language: English
Additional information: Copyright © 2017 Behr et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
URI: http://discovery.ucl.ac.uk/id/eprint/1572540
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