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Oligomeric States In Sodium Ion-Dependent Regulation Of Cyanobacterial Histidine Kinase-2

Allen, J; Ibrahim, IM; Wang, L; Puthiyaveetil, S; Krauß, N; Nield, J; (2017) Oligomeric States In Sodium Ion-Dependent Regulation Of Cyanobacterial Histidine Kinase-2. BioRxiv: Cold Spring Harbor, NY, USA. Green open access

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Abstract

Two-component signal transduction systems (TCSs) consist of sensor histidine kinases and response regulators. TCSs mediate adaptation to environmental changes in bacteria, plants, fungi, and protists. Histidine kinase 2 (Hik2) is a sensor histidine kinase found in all known cyanobacteria and as chloroplast sensor kinase in eukaryotic algae and plants. Sodium ions have been shown to inhibit the autophosphorylation activity of Hik2 that precedes phosphoryl transfer to response regulators, but the mechanism of inhibition has not been determined. We report on the mechanism of Hik2 activation and inactivation probed by chemical crosslinking and size exclusion chromatography together with direct visualisation of the kinase using negative-stain transmission electron microscopy of single particles. We show that the functional form of Hik2 is a higher order oligomer such as a hexamer or octamer. Increased NaCl concentration converts the active hexamer into an inactive tetramer. Furthermore, the action of NaCl appears to be confined to the Hik2 kinase domain.

Type: Working / discussion paper
Title: Oligomeric States In Sodium Ion-Dependent Regulation Of Cyanobacterial Histidine Kinase-2
Open access status: An open access version is available from UCL Discovery
DOI: 10.1101/140145
Publisher version: https://doi.org/10.1101/140145
Language: English
Additional information: The copyright holder for this preprint is the author/funder. It is made available under a CC-BY 4.0 International license (http://creativecommons.org/licenses/by/4.0/).
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment
URI: http://discovery.ucl.ac.uk/id/eprint/1565451
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