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MAARS: a novel high-content acquisition software for the analysis of mitotic defects in fission yeast

Li, T; Mary, H; Grosjean, M; Fouchard, J; Cabello, S; Reyes, C; Tournier, S; (2017) MAARS: a novel high-content acquisition software for the analysis of mitotic defects in fission yeast. Molecular Biology of the Cell , 28 (12) pp. 1601-1611. 10.1091/mbc.E16-10-0723. Green open access

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Abstract

Faithful segregation of chromosomes during cell division relies on multiple processes such as chromosome attachment and correct spindle positioning. Yet mitotic progression is defined by multiple parameters, which need to be quantitatively evaluated. To study the spatiotemporal control of mitotic progression, we developed a high-content analysis (HCA) approach that combines automated fluorescence microscopy with real-time quantitative image analysis and allows the unbiased acquisition of multiparametric data at the single-cell level for hundreds of cells simultaneously. The Mitotic Analysis and Recording System (MAARS) provides automatic and quantitative single-cell analysis of mitotic progression on an open-source platform. It can be used to analyze specific characteristics such as cell shape, cell size, metaphase/anaphase delays, and mitotic abnormalities including spindle mispositioning, spindle elongation defects, and chromosome segregation defects. Using this HCA approach, we were able to visualize rare and unexpected events of error correction during anaphase in wild-type or mutant cells. Our study illustrates that such an expert system of mitotic progression is able to highlight the complexity of the mechanisms required to prevent chromosome loss during cell division.

Type: Article
Title: MAARS: a novel high-content acquisition software for the analysis of mitotic defects in fission yeast
Open access status: An open access version is available from UCL Discovery
DOI: 10.1091/mbc.E16-10-0723
Publisher version: http://doi.org/10.1091/mbc.E16-10-0723
Language: English
Additional information: © 2017 Li et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology
URI: http://discovery.ucl.ac.uk/id/eprint/1564036
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