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Cell culture: complications due to mechanical release of ATP and activation of purinoceptors

Burnstock, G; Knight, GE; (2017) Cell culture: complications due to mechanical release of ATP and activation of purinoceptors. Cell and Tissue Research , 370 (1) pp. 1-11. 10.1007/s00441-017-2618-8. Green open access

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Abstract

There is abundant evidence that ATP (adenosine 5'-triphosphate) is released from a variety of cultured cells in response to mechanical stimulation. The release mechanism involved appears to be a combination of vesicular exocytosis and connexin and pannexin hemichannels. Purinergic receptors on cultured cells mediate both short-term purinergic signalling of secretion and long-term (trophic) signalling such as proliferation, migration, differentiation and apoptosis. We aim in this review to bring to the attention of non-purinergic researchers using tissue culture that the release of ATP in response to mechanical stress evoked by the unavoidable movement of the cells acting on functional purinergic receptors on the culture cells is likely to complicate the interpretation of their data.

Type: Article
Title: Cell culture: complications due to mechanical release of ATP and activation of purinoceptors
Location: Germany
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/s00441-017-2618-8
Publisher version: http://doi.org/10.1007/s00441-017-2618-8
Language: English
Additional information: Copyright © The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.
Keywords: Ectonucleotidases, P1 receptors, P2 receptors, Purinoceptor, Shear stress
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: http://discovery.ucl.ac.uk/id/eprint/1555468
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