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Procedures for cryogenic X-ray ptychographic imaging of biological samples

Yusuf, M; Zhang, F; Chen, B; Bhartiya, A; Cunnea, K; Wagner, U; Cacho-Nerin, F; ... Robinson, IK; + view all (2017) Procedures for cryogenic X-ray ptychographic imaging of biological samples. IUCrJ , 4 (Pt 2) pp. 147-151. 10.1107/S2052252516020029. Green open access

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Abstract

Biological sample-preparation procedures have been developed for imaging human chromosomes under cryogenic conditions. A new experimental setup, developed for imaging frozen samples using beamline I13 at Diamond Light Source, is described. This manuscript describes the equipment and experimental procedures as well as the authors' first ptychographic reconstructions using X-rays.

Type: Article
Title: Procedures for cryogenic X-ray ptychographic imaging of biological samples
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1107/S2052252516020029
Publisher version: http://dx.doi.org/10.1107/S2052252516020029
Language: English
Additional information: This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
Keywords: X-ray ptychography, cell nuclei, chromosome imaging, human chromosomes, plunge freezing
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology
URI: http://discovery.ucl.ac.uk/id/eprint/1554663
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