Kaczara, P;
Motterlini, R;
Kus, K;
Zakrzewska, A;
Abramov, AY;
Chlopicki, S;
(2016)
Carbon monoxide shifts energetic metabolism from glycolysis to oxidative phosphorylation in endothelial cells.
FEBS Letters
, 590
(20)
pp. 3469-3480.
10.1002/1873-3468.12434.
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Abstract
Carbon monoxide (CO) modulates mitochondrial respiration, but the mechanisms involved are not completely understood. The aim of the present study was to investigate the acute effects of CO on bioenergetics and metabolism in intact EA.hy926 endothelial cells using live cell imaging techniques. Our findings indicate that CORM-401, a compound that liberates CO, reduces ATP production from glycolysis, and induces a mild mitochondrial depolarization. In addition, CO from CORM-401 increases mitochondrial calcium and activates complexes I and II. The subsequent increase in mitochondrial respiration leads to ATP production through oxidative phosphorylation. Thus, our results show that nonactivated endothelial cells rely primarily on glycolysis, but in the presence of CO, mitochondrial Ca2+ increases and activates respiration that shifts the metabolism of endothelial cells from glycolysis- to oxidative phosphorylation-dependent ATP production.
| Type: | Article |
|---|---|
| Title: | Carbon monoxide shifts energetic metabolism from glycolysis to oxidative phosphorylation in endothelial cells |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/1873-3468.12434 |
| Publisher version: | http://dx.doi.org/10.1002/1873-3468.12434 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, Biophysics, Cell Biology, carbon monoxide, CO-releasing molecule, endothelium, glycolysis, oxidative phosphorylation, respiration, UNCOUPLES MITOCHONDRIAL RESPIRATION, NITRIC-OXIDE, CYTOCHROME-OXIDASE, MOLECULES, INHIBITION, ATP, ANGIOGENESIS, SUPEROXIDE, PYRUVATE, DISEASE |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Movement Neurosciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1553638 |
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