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A new immunoassay for C3. Application of the cell-linked antigen radioactive antibody (CLARA) technique.

Pepys, MB; Wilson, DV; (1975) A new immunoassay for C3. Application of the cell-linked antigen radioactive antibody (CLARA) technique. J Immunol Methods , 6 (3) pp. 225-233.

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Abstract

The cell-linked antigen radioactive antibody (CLARA) method of immunoassay was applied to the C3 component of the complement system. C3 from whole complement was fixed on erythrocytes sensitized with IgM antibody. The IgG1 fractions of monospecific sheep anti-C3 were labelled with 125I and measurement of their specific binding to C3-bearing erythrocytes was used to quantitate the relative amount of fixed C3. Competititive inhibition of this specific binding was used to assay fluid phase C3. 0.3-0.6mug/ml of murine C3 were detected in the supernatant fluid of in vitro cultures of mouse spleen cells.

Type: Article
Title: A new immunoassay for C3. Application of the cell-linked antigen radioactive antibody (CLARA) technique.
Location: Netherlands
Keywords: Animals, Antigens, Binding, Competitive, Cattle, Complement Fixation Tests, Complement System Proteins, Erythrocytes, Humans, Immune Sera, Immunoassay, Immunoglobulin G, Immunoglobulin M, Iodine Radioisotopes, Mice, Mice, Inbred CBA, Sheep, Spleen, Ultrafiltration
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inflammation
URI: http://discovery.ucl.ac.uk/id/eprint/1552182
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