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Calcium-dependent aggregation of human serum amyloid P component.

Baltz, ML; De Beer, FC; Feinstein, A; Pepys, MB; (1982) Calcium-dependent aggregation of human serum amyloid P component. Biochim Biophys Acta , 701 (2) pp. 229-236. 10.1016/0167-4838(82)90118-2.

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Abstract

Normal human serum or isolated human amyloid P component was ultracentrifuged on density gradients containing either 10 mM EDTA or different concentrations of Ca2+ between 0.15 and 2.15 mM. In the presence of Ca2+ concentrations of 1 mM or more human P component sedimented more rapidly than it did in the presence of lower Ca2+ levels or of EDTA. This phenomenon was due to Ca2+-dependent aggregation of P component molecules and did not require the presence of any other serum constituents. It was completely inhibited by incorporating a physiological concentration (40 mg/ml) of serum albumin in the gradients, suggesting that free ionized Ca2+ is required to promote aggregation of the P component. P component from the mouse and the plaice (Pleuronectes platessa L.), a marine teleost, did not undergo the same Ca2+-dependent aggregation as human P component. These observations resolve a discrepancy existing in the literature concerning the sedimentation rate of human P component in density gradient ultracentrifugation and shed new light on its behaviour with respect to Ca2+ which may be relevant to the deposition of P component in amyloidosis.

Type: Article
Title: Calcium-dependent aggregation of human serum amyloid P component.
Location: Netherlands
DOI: 10.1016/0167-4838(82)90118-2
Keywords: Amyloid, Animals, Calcium, Carrier Proteins, Centrifugation, Density Gradient, Dose-Response Relationship, Drug, Female, Fibronectins, Fishes, Humans, Integrin alphaXbeta2, Macromolecular Substances, Male, Mice, Mice, Inbred CBA, Serum Amyloid P-Component
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inflammation
URI: http://discovery.ucl.ac.uk/id/eprint/1552133
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