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A metagenomics approach for new biocatalyst discovery: application to transaminases and the synthesis of allylic amines

Baud, D; Jeffries, JWE; Moody, TS; Ward, JM; Hailes, HC; (2017) A metagenomics approach for new biocatalyst discovery: application to transaminases and the synthesis of allylic amines. Green Chemistry , 19 (4) pp. 1134-1143. 10.1039/c6gc02769e. Green open access

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Abstract

Transaminase enzymes have significant potential for the sustainable synthesis of amines using mild aqueous reaction conditions. Here a metagenomics mining strategy has been used for new transaminase enzyme discovery. Starting from oral cavity microbiome samples, DNA sequencing and bioinformatics analyses were performed. Subsequent in silico mining of a library of contiguous reads built from the sequencing data identified 11 putative Class III transaminases which were cloned and overexpressed. Several screening protocols were used and three enzymes selected of interest due to activities towards substrates covering a wide structural diversity. Transamination of functionalized cinnamaldehydes was then investigated for the production of valuable amine building blocks.

Type: Article
Title: A metagenomics approach for new biocatalyst discovery: application to transaminases and the synthesis of allylic amines
Open access status: An open access version is available from UCL Discovery
DOI: 10.1039/c6gc02769e
Publisher version: http://doi.org/10.1039/c6gc02769e
Language: English
Additional information: This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Keywords: Science & Technology, Physical Sciences, Chemistry, Multidisciplinary, GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY, Chemistry, Science & Technology - Other Topics, OMEGA-TRANSAMINASES, ASYMMETRIC-SYNTHESIS, FUNCTIONAL METAGENOMICS, SUBSTRATE-SPECIFICITY, PYRUVATE TRANSAMINASE, CHIRAL AMINES, DIVERSITY, ENZYMES, AMINATION, STRATEGY
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
URI: http://discovery.ucl.ac.uk/id/eprint/1549545
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