Movileanu, L and Howorka, S and Braha, O and Bayley, H (2000) Detecting protein analytes that modulate transmembrane movement of a polymer chain within a single protein pore. NAT BIOTECHNOL , 18 (10) 1091 - 1095.
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Abstract
Here we describe a new type of biosensor element for detecting proteins in solution at nanomolar concentrations. We tethered a 3.4 kDa polyethylene glycol chain at a defined site within the lumen of the transmembrane protein pore formed by staphylococcal alpha-hemolysin. The free end of the polymer was covalently attached to a biotin molecule. On incorporation of the modified pore into a lipid bilayer, the biotinyl group moves from one side of the membrane to the other, and is detected by reversible capture with a mutant streptavidin. The capture events are observed as changes in ionic current passing through single pores in planar bilayers. Accordingly, the modified pore allows detection of a protein analyte at the single-molecule level, facilitating both quantification and identification through a distinctive current signature. The approach has higher time resolution compared with other kinetic measurements, such as those obtained by surface plasmon resonance.
| Type: | Article |
|---|---|
| Title: | Detecting protein analytes that modulate transmembrane movement of a polymer chain within a single protein pore |
| Keywords: | biosensor, nanostructure, polymer, pore, protein engineering, STAPHYLOCOCCAL ALPHA-HEMOLYSIN, FORMING PROTEIN, CHANNEL, NANOTECHNOLOGY, STREPTAVIDIN, BINDING, BIOTIN, MUTAGENESIS, RECOGNITION, MOLECULES |
| UCL classification: | UCL > School of BEAMS > Faculty of Maths and Physical Sciences > Chemistry |
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