UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Prickle 1 regulates cell movements during gastrulation and neuronal migration in zebrafish

Carreira-Barbosa, F; Concha, ML; Takeuchi, M; Ueno, N; Wilson, SW; Tada, M; (2003) Prickle 1 regulates cell movements during gastrulation and neuronal migration in zebrafish. DEVELOPMENT , 130 (17) 4037 - 4046.

Full text not available from this repository.

Abstract

During vertebrate gastrulation, mesodermal and ectodermal cells undergo convergent extension, a process characterised by prominent cellular rearrangements in which polarised cells intercalate along the medio-lateral axis leading to elongation of the antero-posterior axis. Recently, it has become evident that a noncanonical Wnt/Frizzled (Fz)/Dishevelled (Dsh) signalling pathway, which is related to the planar-cell-polarity (PCP) pathway in flies, regulates convergent extension during vertebrate gastrulation. Here we isolate and functionally characterise a zebrafish homologue of Drosophila prickle (pk), a gene that is implicated in the regulation of PCP. Zebrafish pk1 is expressed maternally and in moving mesodermal precursors. Abrogation of Pk1 function by morpholino oligonucleotides leads to defective convergent extension movements, enhances the silberblick (slb)1wnt11 and pipetail (Ppt)/wnt5 phenotypes and suppresses the ability of Wnt11 to rescue the slb phenotype. Gain-of-function of Pk1 also inhibits convergent extension movements and enhances the s1b phenotype, most likely caused by the ability of Pk1 to block the Fz7-dependent membrane localisation of Dsh by downregulating levels of Dsh protein. Furthermore, we show that pk1 interacts genetically with trilobite (tri)/strabismus to mediate the caudally directed migration of cranial motor neurons and convergent extension. These results indicate that, during zebrafish gastrulation Pk1 acts, in part, through interaction with the noncanonical Wnt11/Wnt5 pathway to regulate convergent extension cell movements, but is unlikely to simply be a linear component of this pathway. In addition, Pk1 interacts with Tri to mediate posterior migration of branchiomotor neurons, probably independent of the noncanonical Wnt pathway.

Type: Article
Title: Prickle 1 regulates cell movements during gastrulation and neuronal migration in zebrafish
Keywords: Wnt signalling, planar cell polarity, convergent extension, gastrulation, neuronal migration, zebrafish, CONVERGENT EXTENSION MOVEMENTS, 7-PASS TRANSMEMBRANE CADHERIN, DROSOPHILA TISSUE POLARITY, PLANAR POLARITY, WILD-TYPE, VERTEBRATE GASTRULATION, BRANCHIOMOTOR NEURONS, SIGNALING SPECIFICITY, EMBRYONIC-DEVELOPMENT, XENOPUS-LAEVIS
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: http://discovery.ucl.ac.uk/id/eprint/154712
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item